Anti-GSTT2B Polyclonal Antibody for WB, IF/ICC, ELISA - P0CG30

Core Product Specifications and Parameters

Biological Background: GSTT2B Function and Localization

• GSTT2B, also known as GSTT2P, is a member of the glutathione S-transferase theta class and functions as a phase II detoxification enzyme.
• Conjugates reduced glutathione to a wide range of exogenous and endogenous hydrophobic electrophiles, playing a critical role in cellular detoxification, and also possesses sulfatase activity (PubMed:1417752). Related references: PMID:1417752
• The protein is predominantly localized in the cytoplasm and cytosol.
• Tissue expression is generally low, with detectable levels in liver and specific lung cell types including ciliated bronchiolar cells, alveolar macrophages, and alveolar type II cells.
• GSTT2B has a predicted molecular weight of ~28 kDa (27507 Da from sequence) and has been characterized by 3D-structure determination and direct protein sequencing.
• As a reference proteome member, it serves as a model for understanding GST theta-class enzyme mechanisms.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 115–244 of human GSTT2B, targeting the C-terminal region; this may be useful when designing experiments to distinguish closely related isoforms.
• For Western blotting, expect a band at approximately 28 kDa under reducing conditions; confirm specificity using appropriate controls.
• In IF/ICC, optimize fixation and permeabilization conditions to preserve antigenicity, as GSTT2B is a cytosolic protein.
• ELISA can be employed for quantification; consider using recombinant GSTT2B as a standard curve reference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering reliable access to validated antibodies and related reagents for detoxification and phase II enzyme research. From bulk polyclonal antibodies to rare target proteins, CamelBio supports diagnostic manufacturers and labs with end-to-end procurement, technical consultation, and logistical support to streamline development from concept to clinic.