Anti-GSTT2 Rabbit Polyclonal Antibody for WB and ELISA - P0CG29

Core Product Specifications and Parameters

Biological Background: Glutathione S-transferase theta-2 Function and Localization

• GSTT2 (Glutathione S-transferase theta-2), encoded by the GSTT2 gene, belongs to the GST theta family of phase II detoxification enzymes.
• Catalyzes the conjugation of reduced glutathione to a wide range of exogenous and endogenous hydrophobic electrophiles, facilitating their elimination. Related references: PMID:1417752
• Possesses sulfatase activity, enabling the hydrolysis of sulfate ester bonds in certain compounds. Related references: PMID:1417752
• Subcellular localization includes cytoplasm (cytosol) and nucleus, implying functions in both detoxification and nuclear processes.
• Tissue expression is relatively low; detected in liver, and in lung at low levels in ciliated bronchiolar cells, alveolar macrophages, and alveolar type II cells.
• Annotated with keywords: Transferase, Cytoplasm, Nucleus, 3D-structure, Proteomics identification, and Reference proteome, indicating extensive biochemical and structural characterization.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length GSTT2 (amino acids 1-244); the resulting polyclonal antibody is expected to recognize native and denatured forms of the target.
• For Western blot, consider using a starting dilution of 1:500-1:2000 and include positive control lysates from human, mouse, or rat tissues (e.g., liver, lung) known to express GSTT2. The expected band size is approximately 28 kDa.
• For ELISA, the antibody can be employed as a capture or detection reagent; optimization of coating concentration and detector pairing is recommended.
• Validate specificity in the relevant sample system, as cross-reactivity with other GST theta family members may occur.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support glutathione S-transferase research and diagnostic development. With capabilities spanning from concept to clinic, CamelBio streamlines procurement for laboratories and manufacturers requiring reliable supplies for GSTT2 and related detoxification targets.