Anti-GSTT1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30711

Core Product Specifications and Parameters

Biological Background: GSTT1 Function and Localization

• GSTT1 (Glutathione S-transferase theta-1) is a member of the glutathione S-transferase (GST) superfamily, catalyzing the conjugation of reduced glutathione to various hydrophobic electrophiles, playing a critical role in detoxification.
• The enzyme demonstrates activity towards compounds such as 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate, and nitrobenzyl halides, and also possesses selenium-independent glutathione peroxidase activity with cumene hydroperoxide.
• GSTT1 is encoded by a single gene with alternative splicing variants reported, though the predominant isoform is a cytosolic protein of approximately 27kDa.
• The protein localizes primarily to the cytoplasm, consistent with its role in phase II detoxification processes.
• Tissue expression profiling shows GSTT1 is present in erythrocytes and at low levels in the liver; in lung, expression is restricted to club cells and ciliated cells at the alveolar/bronchiolar junction, absent from larger airway epithelial cells.
• Keywords associated with this entry include 3D-structure, alternative splicing, cytoplasm, direct protein sequencing, proteomics identification, reference proteome, and transferase, highlighting its biochemically well-characterized nature.

Experimental Guidance and Technical Tips

• The immunogen covers full-length GSTT1 (amino acids 1-240); thus, the antibody may recognize multiple conformational states. For denaturing Western blot, a band around 27kDa is expected.
• For IHC-P, consider antigen retrieval conditions and validate in tissue types known to express GSTT1 (e.g., liver, lung). Endogenous peroxidase blocking may be required.
• In IF/ICC, cytoplasm staining should be observed; use appropriate fixation and permeabilization methods.
• As a polyclonal antibody, it can provide robust signal but may exhibit batch-to-batch variability; include proper controls and optimize dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with validated IVD raw materials, offering this GSTT1 antibody as part of a comprehensive portfolio for oxidative stress and detoxification studies. Beyond individual antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate assay development from concept to clinical application. Partner with us to streamline your entire IVD supply chain.