Anti-GSTM4 Polyclonal Antibody for WB, ELISA - Q03013

Core Product Specifications and Parameters

Biological Background: GSTM4 Function and Localization

• GSTM4 is a member of the glutathione S-transferase mu class, also designated GST-Mu2, GSTM4-4, and leukotriene C4 synthase GSTM4.
• It catalyzes the conjugation of reduced glutathione to a broad spectrum of exogenous and endogenous hydrophobic electrophiles, playing a key role in detoxification. Related references: PMID:8203914 PMID:8373352
• Specifically, it converts leukotriene A4 to leukotriene C4, an important step in leukotriene biosynthesis. Related references: PMID:27791009
• Additionally, GSTM4 mediates the transfer of glutathione to 13(S),14(S)-epoxy-docosahexaenoic acid, yielding the pro-resolving lipid mediator MCTR1. Related references: PMID:27791009
• Subcellular localization is predominantly cytoplasmic.
• The protein is widely expressed across human tissues.
• The GSTM4 gene undergoes alternative splicing, and the protein structure has been resolved by X-ray crystallography, with direct protein sequencing evidence available.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant GSTM4 protein (aa 1-218), which is likely to present native epitopes; consider this when selecting detection probes.
• For Western blot, validate in your sample system; the predicted molecular weight is 26 kDa under reducing conditions.
• ELISA can be employed for quantitative analysis; optimal antibody pairs should be screened empirically.
• Based on cross-reactivity data, the antibody may recognize both human and mouse GSTM4; sequence alignment is recommended before extending to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support, streamlining your development from concept to clinic. This anti-GSTM4 polyclonal antibody supports research in detoxification, leukotriene signaling, and inflammation. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your diagnostic applications.