Anti-GSTM3 Polyclonal Antibody for WB, ELISA - P21266

Core Product Specifications and Parameters

Biological Background: GSTM3 Function and Localization

• GSTM3 is a glutathione S-transferase that catalyzes the conjugation of reduced glutathione to various exogenous and endogenous hydrophobic electrophiles, playing a key role in cellular detoxification.
• It may be involved in governing the uptake and detoxification of compounds at the testis and brain blood barriers.
• GSTM3 belongs to the Mu class of glutathione S-transferases and is also known as GST class-mu 3 (GSTM3-3).
• The protein is primarily localized in the cytoplasm.
• It is expressed in a tissue-specific manner, with notable presence in testis and brain.
• Post-translational modifications include isopeptide bond formation and ubiquitin-like conjugation, as indicated by UniProt keywords.
• The mature protein has a molecular weight of approximately 26.6 kDa, with an apparent weight around 27 kDa in assays.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 101-225 of human GSTM3, a region that may be conserved between human and mouse, supporting the observed cross-reactivity.
• For Western blotting (WB), consider using a starting dilution range typical for polyclonal antibodies and validate in relevant tissue lysates (e.g., testis or brain) where GSTM3 is expressed.
• In ELISA applications, ensure the antibody is validated in the appropriate sample matrix, as the antibody is a polyclonal and may exhibit lot-to-lot variability.
• The antibody detects both human and mouse GSTM3; however, cross-reactivity with other species should be verified experimentally.
• The shipping condition (ice bag) suggests low-temperature transport; upon arrival, store as recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive sourcing solution for IVD raw materials. This anti-GSTM3 polyclonal antibody supports research into detoxification pathways and barrier functions, and is available alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. CamelBio specializes in rapidly sourcing even rare target raw materials, bridging the gap from concept to clinic.