Anti-GSTM1 Polyclonal Antibody for WB, ELISA - P09488

Core Product Specifications and Parameters

Biological Background: GSTM1 Function and Localization

• GSTM1 (glutathione S-transferase Mu 1) is a cytosolic enzyme that catalyzes the conjugation of reduced glutathione to various electrophilic compounds, playing a key role in detoxification.
• It is involved in the metabolism of prostaglandins, specifically forming glutathione conjugates of prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Related references: PMID:9084911
• GSTM1 participates in the biosynthesis of hepoxilin regioisomers, which are lipid mediators involved in inflammation and other processes (PubMed:21046276). Related references: PMID:21046276
• Localized in the cytoplasm, GSTM1 is highly expressed in the liver, as detected at the protein level.
• The gene is subject to alternative splicing, producing multiple isoforms (GSTM1a-1a, GSTM1b-1b), and is classified as a transferase involved in lipid metabolism.
• Post-translational modifications include phosphorylation, and the protein has an observed molecular weight of approximately 26 kDa (calculated 25.7 kDa).

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 1-181 of human GSTM1, covering the full-length protein. For WB, a band around 26 kDa is expected in lysates from human, mouse, and rat tissues; liver samples may yield the strongest signal.
• For ELISA, consider using recombinant GSTM1 or cell lysates as coating antigen; validate with appropriate positive and negative controls.
• Since GSTM1 exists in multiple isoforms, verify specificity by consulting sequence alignments if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. For research targeting glutathione metabolism and detoxification pathways, the GSTM1 antibody facilitates studies on oxidative stress and lipid mediator biology. With our one-stop platform, diagnostic manufacturers and researchers can access rare targets and technical support from concept to clinic.