Anti-GSTK1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y2Q3

Core Product Specifications and Parameters

Biological Background: GSTK1 Function and Localization

• Glutathione S-transferase kappa 1 (GSTK1) is a member of the glutathione S-transferase (GST) superfamily, also designated as GST class-kappa or hGSTK1.
• GSTK1 catalyzes the conjugation of reduced glutathione to various hydrophobic and electrophilic compounds, contributing to cellular detoxification. Significant catalytic activity has been observed using the model substrate 1-chloro-2,4-dinitrobenzene (CDNB). Related references: PMID:14709161 PMID:14742434
• Unlike the majority of cytosolic GSTs, GSTK1 localizes to peroxisomes, indicating a specialized role in organelle-specific detoxification and metabolism.
• Protein expression is ubiquitous across tissues, reflecting its fundamental importance in maintaining cellular redox homeostasis.
• Post-translational modifications, including acetylation, have been documented, and alternative splicing generates multiple isoforms, suggesting complex regulatory mechanisms.
• The three-dimensional structure of GSTK1 has been solved, providing insights into its substrate-binding and catalytic properties.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the full-length human GSTK1 protein (amino acids 1–226), which may enable detection of multiple splice isoforms if present.
• For immunoblotting (WB), an expected band around 25 kDa should be observed; loading controls and molecular weight markers are recommended.
• Given the peroxisomal localization, immunofluorescence and immunohistochemistry protocols may require optimized permeabilization steps to achieve clear organellar staining.
• This antibody has been shown to cross-react with mouse and rat GSTK1, but validation in the end-user’s species and tissue of interest is advised.
• While suitable for ELISA, pairing with a matched detection antibody and titration experiments are necessary for quantitative assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, CROs, and research laboratories with comprehensive access to IVD-grade raw materials and tailored sourcing solutions. This anti-GSTK1 antibody supports research into peroxisomal function, detoxification pathways, and redox regulation. Beyond individual antibodies, CamelBio streamlines procurement of validated antibody pairs, bulk monoclonals/polyclonals, high-quality ancillary reagents, and rare-target raw materials—enabling you to advance from concept to clinical application with confidence.