Anti-GSTK1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2Q3

Core Product Specifications and Parameters

Biological Background: GSTK1 Function and Localization

• Glutathione S-transferase kappa 1 (GSTK1, also known as hGSTK1, GST13-13, GST class-kappa) is a member of the glutathione S-transferase family that catalyzes the conjugation of glutathione to exogenous and endogenous compounds, with significant activity towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB).
  Related references: PMID:14709161, PMID:14742434
• GSTK1 localizes to the peroxisome, distinguishing it from most cytosolic GSTs, and may play a role in peroxisomal metabolism and redox regulation.
• The protein is ubiquitously expressed across tissues, suggesting a housekeeping function in cellular detoxification processes.
• Post-translational modifications include acetylation, and alternative splicing adds regulatory diversity; the 3D structure of GSTK1 has been determined (keyword).
• Classified as a transferase, GSTK1 is part of the reference proteome and has been identified in multiple proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GSTK1 protein (aa 1-226); in Western blot (WB), expect a band at ~25 kDa. Validate loading controls and optimize blocking conditions for low-molecular-weight targets.
• For immunofluorescence (IF/ICC), consider permeabilization and fixation protocols suitable for peroxisomal staining, and confirm specificity using GSTK1-overexpressing cells if available.
• In ELISA applications, the polyclonal antibody can be employed as either a capture or detection reagent; cross-reactivity with mouse and rat orthologs may support multi-species assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by providing comprehensive access to IVD raw materials, including validated antibodies for targets like GSTK1 involved in detoxification and metabolic disease research. Beyond this polyclonal antibody, we offer optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents for assay development. For specialized needs, CamelBio also facilitates rare-target raw-material sourcing, ensuring you can move confidently from concept to clinical application.