Anti-GSTK1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2Q3

Core Product Specifications and Parameters

Biological Background: GSTK1 Function and Localization

• Glutathione S-transferase kappa 1 (GSTK1) is an enzyme that catalyzes the conjugation of reduced glutathione to a variety of exogenous and endogenous hydrophobic electrophiles.
• It shows significant conjugating activity primarily with the model substrate 1-chloro-2,4-dinitrobenzene (CDNB). Related references: PMID:14709161, PMID:14742434
• GSTK1 belongs to the glutathione S-transferase kappa class and is also known as GST 13-13, GST class-kappa, GSTK1-1, and glutathione S-transferase subunit 13.
• The protein localizes predominantly to peroxisomes.
• GSTK1 is ubiquitously expressed across human tissues.
• It has a molecular weight of approximately 25.5 kDa (25497 Da as per UniProt).
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing. It is part of the reference proteome with a known 3D structure.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein encoding the full-length human GSTK1 (a.a. 1–226); it is expected to recognize the native and denatured forms of the protein in assays such as WB.
• For Western blot, a band at ~25 kDa should be detected in human cell or tissue lysates; knockdown or knockout controls are recommended to confirm specificity.
• In immunofluorescence/immunocytochemistry (IF/ICC), appropriate permeabilization is required to access the peroxisomal GSTK1; optimize fixation and permeabilization conditions for your cell type.
• For ELISA applications, the antibody may serve as a capture or detection reagent; pair with a matched antibody for sandwich ELISA development.
• The antibody has been validated by knockout (KO) in the supplier's quality control; consider independent validation in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing hub for IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to validated antibodies, antigens, and ancillary reagents. This GSTK1 polyclonal antibody exemplifies our catalog for targets involved in oxidative stress, detoxification, and peroxisomal biology. In addition to individual antibodies, we provide custom bulk orders, antibody pair optimization, and rare target procurement to support your assay development from concept to clinic.