Anti-GSTK1 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9Y2Q3

Core Product Specifications and Parameters

Biological Background: GSTK1 Function and Localization

• GSTK1 (Glutathione S-transferase kappa 1), also referred to as hGSTK1, GST 13-13, or GST class-kappa, is a member of the glutathione S-transferase superfamily (Gene: 373156).
• This enzyme conjugates glutathione to a range of substrates, with significant catalytic activity observed only with the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) according to published studies. Related references: PMID:14709161 PMID:14742434
• GSTK1 localizes predominantly to peroxisomes.
• The protein shows ubiquitous tissue expression.
• Alternative splicing produces multiple isoforms; available 3D structural data and proteomic evidence support its classification as a transferase.
• Post-translational modifications include acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human GSTK1; users may consider that this region could detect multiple splice variants.
• For Western blot, validate in both human and mouse lysates given the reported cross-reactivity; a band around 25 kDa is expected.
• In immunofluorescence/ICC, a peroxisomal staining pattern is anticipated; appropriate controls should be included.
• For ELISA, consider the accessibility of the epitope and potential matrix effects; empirical optimization of coating and blocking conditions is recommended.

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CamelBio offers diagnostic manufacturers and research laboratories comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies such as this anti-GSTK1 clone. Our portfolio encompasses bulk ancillary reagents and rare target sourcing to support your assay development from concept to clinic. Explore our catalog for high-quality reagents for glutathione metabolism and peroxisomal research.