Anti-GSTA3 Rabbit Polyclonal Antibody for WB, IF, ELISA - Q16772

Core Product Specifications and Parameters

Biological Background: GSTA3 Function and Localization

• Glutathione S-transferase A3 (GSTA3), also known as GST class-alpha member 3 or GSTA3-3, is a member of the alpha class glutathione S-transferase family, encoded by the GSTA3 gene on human chromosome 6.
• GSTA3 catalyzes the conjugation of reduced glutathione to a wide variety of exogenous and endogenous hydrophobic electrophiles, playing a critical role in cellular detoxification and defense against oxidative stress.
• A specialized function of GSTA3 is the isomerization of double bonds in steroid precursors; it efficiently catalyzes the obligatory isomerizations of delta(5)-androstene-3,17-dione and delta(5)-pregnene-3,20-dione, contributing to testosterone and progesterone biosynthesis, respectively.
• The enzyme localizes predominantly to the cytoplasm, consistent with its role in phase II metabolism and steroidogenic pathways.
• GSTA3 is subject to post-translational acetylation, as indicated by proteomic studies, and is annotated as a transferase involved in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human GSTA3 protein (aa 1-222), which is expected to elicit antibodies recognizing the native protein in various sample types.
• For Western blot (WB), the predicted molecular weight is ~25 kDa; consider optimizing loading controls and primary antibody dilution to minimize background.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate a cytoplasmic staining pattern; include appropriate blocking and permeabilization steps.
• For ELISA applications, validate the antibody’s performance in the relevant matrix with known positive and negative controls.
• Cross-reactivity is reported with human, mouse, and rat; verify species specificity if working with other model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between raw material discovery and diagnostic kit commercialization, offering a curated portfolio of IVD-grade antibodies and reagents. For research and assay development focused on GSTA3—a key player in detoxification and steroidogenesis—we supply validated polyclonal antibodies, optimized antibody pairs, and bulk ancillary materials. Whether you need rare-target sourcing or end-to-end technical consulting, CamelBio supports your pipeline from concept to clinic.