Anti-GSTA1 Rabbit Polyclonal Antibody for WB, ELISA - P08263

Core Product Specifications and Parameters

Biological Background: GSTA1 Function and Localization

• Glutathione S-transferase A1 (GSTA1), also known as GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, and GTH1, is a 25631 Da protein (UniProt) belonging to the GST-alpha family.
• GSTA1 conjugates reduced glutathione to electrophilic xenobiotics and endogenous compounds, playing a critical role in cellular detoxification (Probable).
• Specifically, it catalyzes the formation of glutathione conjugates of prostaglandin A2 (PGA2) and J2 (PGJ2). Related references: PMID:9084911
• GSTA1 also exhibits steroid isomerase activity, converting androst-5-ene-3,17-dione to androst-4-ene-3,17-dione, implicating it in hormone biosynthesis. Related references: PMID:11152686
• Through its glutathione-dependent peroxidase activity, GSTA1 reduces fatty acid hydroperoxides such as 13-HPODE, contributing to lipid metabolism and oxidative stress defense. Related references: PMID:16624487
• GSTA1 is predominantly localized in the cytoplasm.
• It is highly expressed in the liver.
• Keywords associated with GSTA1 include: Acetylation, Cytoplasm, Direct protein sequencing, Isomerase, Lipid metabolism, Oxidoreductase, Peroxidase, Reference proteome, Transferase.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to full-length human GSTA1 (amino acids 1–222). For Western blot analysis, a band around 26 kDa is expected; validate with lysates from human or rat liver.
• For ELISA, consider pairing with a matched detection antibody and optimizing coating conditions to improve signal-to-noise ratio.
• Pre-absorption with the immunogen may help confirm specificity; consider using GSTA1 knockout cell lines or tissue controls.
• Ensure end‑user validation in the target experimental system, as cross-reactivity with other GST isoforms is possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, providing this GSTA1 rabbit polyclonal antibody along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our expertise spans detoxification and hormone biosynthesis research areas, ensuring reliable supply for diagnostic development.