Anti-GST3/GSTP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09211

Core Product Specifications and Parameters

Biological Background: GSTP1 Function and Localization

• GSTP1 (Glutathione S-transferase P, also known as GST class-pi or GSTP1-1) is a member of the glutathione S-transferase family that catalyzes glutathione conjugation to electrophilic compounds. Related references: PMID:1540159, PMID:1567427, PMID:8433974
• It participates in the metabolism of prostaglandins A2 and J2 via glutathione conjugation. Related references: PMID:9084911
• Involved in the formation of hepoxilin regioisomers, expanding the spectrum of lipid mediators. Related references: PMID:21046276
• Acts as a negative regulator of ferroptosis by mediating glutathione-dependent detoxification of 4-hydroxynonenal (4-HNE). Related references: PMID:38016474
• Negatively regulates CDK5 activity by controlling p25/p35 translocation, contributing to neuroprotection. Related references: PMID:21668448
• Localizes to the cytoplasm, mitochondria, and nucleus, reflecting its diverse functional roles.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitination; it is also known for 3D-structure determination and lipid metabolism involvement.
• Alias names include PI, DFN7, GST3, GSTP, FAEES3, and HEL-S-22, indicative of its broad biological contexts.

Experimental Guidance and Technical Tips

• For Western blotting, consider using the predicted molecular weight of ~23 kDa as a reference.
• Due to the polyclonal nature of the antibody, it is recommended to validate batch-to-batch consistency and use appropriate positive and negative controls.
• For IHC-P and IF/ICC, optimize fixation, permeabilization, and antigen retrieval conditions empirically for your specific tissue or cell type.
• When working with mouse samples, confirm reactivity under your experimental conditions and consider species-specific blocking strategies if needed.
• Dilution factors for each application should be determined experimentally; start with a range suggested by standard protocols for polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, offering one-stop access to IVD raw materials. This anti-GSTP1 antibody supports investigations into glutathione metabolism, ferroptosis, and oxidative stress-related pathways. Beyond single reagents, we can facilitate procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing to streamline your project from concept to clinic.