Anti-GSR Rabbit Monoclonal Antibody for WB, ELISA - P00390

Core Product Specifications and Parameters

Biological Background: GSR Function and Localization

• Glutathione reductase (GSR) is a flavoprotein that catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is crucial for maintaining cellular redox homeostasis.
• GSR is a member of the NADP-dependent oxidoreductase family and contains a redox-active disulfide center and an FAD prosthetic group.
• The protein is encoded by the GSR gene (Gene ID: 2936) and undergoes alternative splicing and alternative initiation, resulting in multiple isoforms.
• Subcellular localization studies indicate GSR is primarily mitochondrial, but it is also found in the cytoplasm.
• Post-translational modifications such as acetylation and disulfide bond formation may regulate enzyme activity.
• Mutations in GSR are associated with hereditary hemolytic anemia, highlighting its essential role in protecting erythrocytes from oxidative damage.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (aa 423-522) of human GSR; this region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, expect a band at approximately 56 kDa under reducing conditions; validate in your target species and tissue, as expression levels may vary.
• For ELISA, this antibody may be used for direct or indirect detection of GSR in cell lysates or purified protein; consider optimizing coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic developers a curated selection of high-quality IVD raw materials, including validated monoclonal antibodies like this Anti-GSR Rabbit mAb. As your one-stop sourcing bridge, we provide antibody pairs, bulk ancillary reagents, and access to rare targets to support your assay development and research in redox biology and oxidative stress.