Anti-GSPT1 Rabbit Polyclonal Antibody for WB, ELISA - P15170

Core Product Specifications and Parameters

Biological Background: GSPT1 Function and Localization

• Also known as ERF3A (Eukaryotic peptide chain release factor GTP-binding subunit ERF3A) and G1 to S phase transition protein 1 homolog, GSPT1 is encoded by the GSPT1 gene (Gene ID: 2935).
• GSPT1 is the GTPase component of the eRF1-eRF3-GTP ternary complex, which mediates translation termination at UAA, UAG, and UGA stop codons by delivering eRF1 to the ribosomal A-site. Related references: PMID:15987998, PMID:19417105, PMID:2511002, PMID:27863242, PMID:16777602
• In nonsense-mediated mRNA decay (NMD), GSPT1 is a component of the transient SURF complex that recruits UPF1 to stalled ribosomes, targeting mRNAs with premature stop codons for degradation. Related references: PMID:24486019
• Required for SHFL-mediated translation termination, which inhibits programmed ribosomal frameshifting (-1PRF) of viral and cellular mRNAs, highlighting a role in antiviral defense. Related references: PMID:30682371
• Classified by UniProt keywords as a GTP-binding protein, hydrolase, and nucleotide-binding protein; also identified in the reference proteome and subject to alternative splicing.
• UniProt lists a molecular weight of approximately 56 kDa for the canonical isoform, consistent with the 56kDa band observed in western blotting.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 138–420 of human GSPT1, a region encompassing the GTP-binding domain; this design may favor recognition of the native protein and contribute to the reported cross‑reactivity with mouse and rat.
• For western blot (WB), consider that the predicted molecular weight is ~56 kDa; use appropriate gel percentages and loading controls to confirm specificity in your sample type.
• ELISA protocols may require optimization of coating antigen and antibody titration; include a known positive control (e.g., recombinant GSPT1 protein) to validate assay performance.
• Because GSPT1 is evolutionarily conserved, cross‑reactivity across human, mouse, and rat is expected, but we recommend verifying reactivity in your model system with appropriate species‑matched secondary antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supplier of IVD raw materials and technical services, supporting diagnostic manufacturers and research laboratories from early development through to commercial assay production. This anti-GSPT1 polyclonal antibody is part of our expanding catalog for translation termination and RNA surveillance research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare‑target biomaterials—all designed to streamline your workflow and accelerate time‑to‑result.