Anti-GSK3β Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P49841

Core Product Specifications and Parameters

Biological Background: GSK3β Function and Localization

• GSK3β (GSK3B; gsk-3β) is a constitutively active serine/threonine-protein kinase that negatively regulates glycogen synthase (GYS1/GYS2), translation initiation factor EIF2B, Wnt pathway components (CTNNB1/beta-catenin, APC, AXIN1), and microtubule-associated proteins (MAPT/TAU, MACF1). Related references: PMID:11430833 PMID:12554650 PMID:14690523
• In insulin signaling, GSK3β phosphorylates and inhibits GYS1, thereby regulating glycogen synthesis; it also controls EIF2B activity, modulating protein synthesis. Related references: PMID:8397507
• In the Wnt pathway, GSK3β forms a complex with APC, AXIN1, and beta-catenin, phosphorylating the N-terminus of beta-catenin and targeting it for ubiquitin-proteasome degradation. Related references: PMID:12554650
• GSK3β phosphorylates multiple transcription factors, including JUN (reducing its DNA affinity), NFATC (promoting nuclear export), and SNAI1 (promoting its degradation). It also regulates the circadian clock by phosphorylating BMAL1, CLOCK, and PER2. Related references: PMID:1846781 PMID:9072970 PMID:15448698 PMID:15647282 PMID:25827072 PMID:19946213 PMID:28903391
• In neurons, GSK3β is essential for polarity establishment and axon outgrowth, and it phosphorylates MAPT/TAU on 'Thr-548', reducing microtubule binding; hyperphosphorylated TAU is a hallmark of Alzheimer’s disease. Related references: PMID:20067585 PMID:14690523
• GSK3β participates in autophagy regulation by phosphorylating KAT5/TIP60 under starvation, promoting acetylation of key autophagy regulators; it also modulates apoptosis through an anti-apoptotic complex with DDX3X and BRIC2 at death receptors. Related references: PMID:30704899 PMID:18846110
• Subcellularly localized to the cytoplasm, nucleus, and cell membrane, GSK3β undergoes multiple post-translational modifications (phosphorylation, ubiquitination, palmitoylation) and is associated with diabetes mellitus, Alzheimer's disease, and circadian rhythms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 320-420 in the C-terminal region of human GSK3β; this region is conserved in mouse and rat, consistent with the observed cross-reactivity.
• For Western blot (WB), the predicted molecular weight is 47 kDa. Phosphorylation or alternative splicing may cause band shifts; consider using phosphatase inhibitors or verifying with a loading control.
• GSK3β is a pleiotropic kinase; validate detection in your specific cell or tissue model using known inhibitors (e.g., CHIR99021) or siRNA knockdown.
• For immunofluorescence (IF/ICC), epitope accessibility can vary with fixation method; test methanol vs. paraformaldehyde permeabilization.
• Polyclonal antibodies can provide robust signal in IP and ELISA, but batch-to-batch consistency should be confirmed for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, clinical labs, and research institutes with comprehensive IVD raw-material solutions, technical support, and consulting—covering every stage from concept to clinic. For GSK3β and related kinase targets, we offer validated polyclonal and monoclonal antibodies, purified antigens, and bulk ancillary reagents to accelerate your assay development and production. Partner with us for reliable, scalable supply tailored to your project needs.