Anti-GSK3β Monoclonal Antibody for WB, IP, IF-P, ELISA - P49841

Core Product Specifications and Parameters

Biological Background: GSK3β Function and Localization

• Glycogen synthase kinase-3 beta (GSK3β) is a constitutively active serine/threonine-protein kinase, encoded by the GSK3B gene, that regulates glucose homeostasis, Wnt signaling, and microtubule dynamics.
• In insulin signaling, GSK3β phosphorylates and inhibits glycogen synthase (GYS1), reducing glycogen synthesis, and controls protein synthesis via EIF2B phosphorylation (PubMed:8397507). Related references: PMID:8397507
• As a key component of the Wnt signaling pathway, GSK3β forms a destruction complex with APC, AXIN1, and β-catenin, phosphorylating β-catenin for ubiquitin-mediated degradation (PubMed:12554650). Related references: PMID:12554650
• GSK3β phosphorylates microtubule-associated protein tau (MAPT) at Thr-548, reducing tau's ability to stabilize microtubules, and is implicated in Alzheimer's disease neurofibrillary tangles (PubMed:14690523). Related references: PMID:14690523
• It regulates the circadian clock by phosphorylating BMAL1 and CLOCK, targeting them for proteasomal degradation, thereby influencing circadian rhythmicity (PubMed:19946213, PubMed:28903391). Related references: PMID:19946213 PMID:28903391
• GSK3β is localized in the cytoplasm, nucleus, and cell membrane, and is expressed in diverse tissues including testis, thymus, prostate, and ovary, with lower expression in brain and kidney.
• The protein undergoes multiple post-translational modifications, including phosphorylation, palmitoylation, and ADP-ribosylation, and plays roles in neurogenesis, development, and cell differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 321–420) of human GSK3β, which may contribute to high specificity for the target.
• In Western blot (WB), the antibody detects a band around 47 kDa, consistent with the predicted molecular weight; appropriate loading controls and molecular weight markers should be used.
• For immunoprecipitation (IP), optimize antibody concentration and incubation conditions to ensure efficient capture of GSK3β from cell lysates.
• For immunofluorescence (IF-P), consider fixation/permeabilization methods compatible with the subcellular distribution of GSK3β (cytoplasmic, nuclear, and membrane).
• Cross-reactivity with mouse and rat GSK3β supports use in rodent models; verify expression levels in target tissues.
• Validation in the relevant sample system (e.g., cell lines or primary tissues expressing GSK3β) is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering validated antibodies like this anti-GSK3β monoclonal antibody for research in metabolic disorders, oncology, and neurodegenerative diseases. We support diagnostic developers with optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing solutions for rare targets, ensuring seamless transition from concept to clinical application.