Anti-GSK3α Rabbit Monoclonal Antibody [KO Validated] for WB, IP, ELISA - P49840

Core Product Specifications and Parameters

Biological Background: GSK3α Function and Localization

• GSK3α is a constitutively active serine/threonine kinase that negatively regulates glycogen synthase (GYS1/GYS2), CTNNB1/beta-catenin, APC, and AXIN1 by phosphorylation, requiring primed substrate phosphorylation. It contributes to insulin regulation of glycogen synthesis in liver. Related references: PMID:11749387, PMID:17478001, PMID:19366350
• May mediate insulin resistance by regulating transcription factors. Related references: PMID:10868943, PMID:17478001
• In Wnt signaling, GSK3α regulates nuclear CTNNB1/beta-catenin levels and transcriptional activity. Related references: PMID:17229088
• Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques involved in Alzheimer disease. Related references: PMID:12761548
• Acts as a regulator of autophagy by phosphorylating KAT5/TIP60 under starvation, promoting acetylation of ULK1 and RUBCNL/Pacer. Related references: PMID:30704899
• Phosphorylates mTORC2 component RICTOR at Thr-1695, leading to FBXW7-mediated ubiquitination and degradation. Related references: PMID:25897075
• Necessary for neuronal polarity and axon outgrowth.
• Negatively regulates extrinsic apoptosis and may control cell apoptosis via MCL1 phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100). For Western blot, the predicted molecular weight is 51 kDa; validate with appropriate positive and negative controls.
• For IP and ELISA, consider using standard protocols with anti-rabbit secondary antibodies.
• Cross-reactivity with human, mouse, rat implies conserved epitopes; verify in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material supplier providing validated antibodies, bulk reagents, and rare target sourcing. For GSK3α research in Wnt signaling, metabolism, or Alzheimer's, we offer monoclonal and polyclonal antibody options, along with technical consulting. Our comprehensive portfolio supports diagnostic developers from concept to clinic.