Anti-GSK3α Polyclonal Antibody for WB - P49840

Core Product Specifications and Parameters

Biological Background: GSK3α Function and Localization

• Glycogen synthase kinase-3 alpha (GSK3α) is a constitutively active serine/threonine-protein kinase encoded by the GSK3A gene (UniProt P49840). It has a molecular weight of approximately 51 kDa and belongs to the GSK3 family. Related references: PMID:11749387, PMID:17478001, PMID:19366350

• GSK3α negatively regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase (GYS1 and GYS2), thereby playing a key role in insulin-mediated glucose homeostasis. Related references: PMID:11749387, PMID:17478001, PMID:19366350

• In the Wnt signaling pathway, GSK3α phosphorylates CTNNB1/beta-catenin, APC, and AXIN1, promoting beta-catenin degradation and acting as a negative regulator of the pathway. Related references: PMID:17229088

• It contributes to Alzheimer disease pathology by facilitating amyloid precursor protein (APP) processing and generation of amyloid plaques. Related references: PMID:12761548

• GSK3α regulates autophagy by phosphorylating KAT5/TIP60 under starvation, which enhances KAT5 acetyltransferase activity and promotes acetylation of ULK1 and RUBCNL/Pacer. Related references: PMID:30704899

• Phosphorylates RICTOR at Thr-1695, leading to FBXW7-mediated ubiquitination and degradation, thus modulating mTORC2 signaling. Related references: PMID:25897075

• GSK3α is involved in the establishment of neuronal polarity and axon outgrowth, and may regulate pancreatic beta-cell replication.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 400-483 at the C-terminus of human GSK3α. For Western blot, expect a major band near 51 kDa. Validate specificity using positive controls like HeLa or HEK293 cell lysates, as the antibody cross-reacts with human, mouse, and rat.
• For ELISA applications, consider an indirect ELISA format using the immunogen peptide as coating antigen or recombinant GSK3α protein. Include appropriate negative controls to assess background.
• For WB, start with a primary antibody dilution range of 1:500 to 1:2000 and optimize based on your sample type and detection system. Ensure proper blocking to reduce non-specific binding.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies using rodent models. However, always confirm expression and molecular weight in your specific tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing, supporting your metabolic and neurodegenerative disease research with validated antibodies and critical reagents. This anti-GSK3α polyclonal antibody is part of our extensive portfolio for studying Wnt signaling, insulin resistance, and Alzheimer's disease. Partner with us for bulk antibody supply, custom development, and ancillary reagents to accelerate your diagnostic assay pipeline.