Anti-GSK-3α/β Rabbit Polyclonal Antibody for IHC-P, ELISA - P49840 / P49841

Core Product Specifications and Parameters

Biological Background: GSK-3α/β Function and Localization

• GSK-3α is a constitutively active serine/threonine kinase that acts as a negative regulator in insulin and Wnt signaling pathways by phosphorylating key substrates like glycogen synthase (GYS1/GYS2), CTNNB1 (β-catenin), APC, and AXIN1. Related references: PMID:11749387, PMID:17478001, PMID:19366350
• It plays a role in glycogen metabolism, specifically regulating glycogen synthesis in the liver, and may contribute to insulin resistance by modulating transcription factors. Related references: PMID:10868943, PMID:17478001
• In Wnt signaling, GSK-3α regulates the stability and transcriptional activity of β-catenin, influencing cell proliferation and differentiation. Related references: PMID:17229088
• GSK-3α facilitates amyloid precursor protein (APP) processing and the formation of amyloid plaques, linking it to Alzheimer disease pathology. Related references: PMID:12761548
• It phosphorylates RICTOR at Thr-1695, promoting FBXW7-mediated ubiquitination and degradation of the mTORC2 component, impacting cell growth and metabolism. Related references: PMID:25897075
• Under starvation conditions, GSK-3α phosphorylates KAT5/TIP60, enhancing acetyltransferase activity and promoting autophagy regulators like ULK1 and RUBCNL/Pacer. Related references: PMID:30704899
• GSK-3α is subject to multiple post-translational modifications, including phosphorylation and acetylation, which regulate its activity and interactions.
• The antibody detects GSK-3α and GSK-3β isoforms migrating at approximately 46 kDa and 48 kDa, respectively, in human and mouse samples, making it a versatile tool for studying GSK-3 signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa 253-368) shared between GSK-3α and GSK-3β, ensuring recognition of both isoforms in human and mouse.
• For IHC-P applications, consider optimizing antigen retrieval conditions (e.g., citrate buffer pH 6.0) and antibody dilution to achieve specific staining.
• In ELISA, validate coating and detection parameters using the recommended antigen format and appropriate blocking reagents to minimize nonspecific binding.
• Confirm lot-to-lot consistency and species-specific reactivity by including suitable positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, supporting diagnostic development from concept to clinic. This anti-GSK-3α/β antibody is an excellent choice for research in metabolic disorders, oncology, and neurodegeneration. For bulk orders, customized sourcing, or technical guidance, reach out to our team.