Anti-GSK-3α/β Monoclonal Antibody for WB, IP, ELISA - P49840 / P49841

Core Product Specifications and Parameters

Biological Background: GSK-3α/β Function and Localization

• GSK-3α (encoded by GSK3A) is a constitutively active serine/threonine kinase that serves as a critical negative regulator in multiple pathways including glycogen synthesis, Wnt/β-catenin signaling, and transcriptional regulation (PubMed:11749387, PubMed:17478001, PubMed:19366350). Related references: PMID:11749387 PMID:17478001 PMID:19366350
• In insulin-regulated glucose homeostasis, GSK-3α phosphorylates and inactivates glycogen synthase (GYS1), thereby inhibiting glycogen synthesis; this mechanism is central to metabolic control in the liver (PubMed:11749387, PubMed:17478001, PubMed:19366350). Related references: PMID:11749387 PMID:17478001 PMID:19366350
• Within the canonical Wnt pathway, GSK-3α forms part of the destruction complex targeting β-catenin for degradation; its inhibition leads to β-catenin stabilization and nuclear signaling (PubMed:17229088). Related references: PMID:17229088
• GSK-3α also contributes to Alzheimer's disease pathology by facilitating amyloid precursor protein (APP) processing and the generation of neurotoxic amyloid-β plaques (PubMed:12761548). Related references: PMID:12761548
• Under starvation conditions, GSK-3α regulates autophagy by phosphorylating KAT5/TIP60, enhancing its acetyltransferase activity toward key autophagy regulators such as ULK1 (PubMed:30704899). Related references: PMID:30704899
• Additionally, GSK-3α negatively regulates extrinsic apoptotic signaling by forming an anti-apoptotic complex at death receptors, with effects that depend on signal strength.
• This antibody also recognizes GSK-3β (encoded by GSK3B), a highly homologous isoform with parallel roles in glycogen metabolism and Wnt signaling.
• Key functional keywords: Alzheimer disease, diabetes mellitus, glycogen metabolism, Wnt signaling pathway, autophagy, apoptosis.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant human GSK-3α/β (NP_063937.2), suggesting that the antibody targets conserved epitopes across both isoforms; researchers should validate isoform specificity in their model.
• For Western blot (WB), lysates from human, mouse, or rat cell lines may reveal bands at approximately 46 kDa (GSK-3α) and 48 kDa (GSK-3β). Use appropriate gel concentrations and loading controls.
• Immunoprecipitation (IP) and ELISA applications are listed; optimization of antibody concentration and buffer conditions is recommended for specific experimental setups.
• When studying signaling pathways (e.g., Wnt or insulin), treat cells with pathway activators/inhibitors and monitor phosphorylation status of downstream targets.
• Storage and handling: upon receipt, store at -20°C; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For GSK-3α/β-related assay development, we offer validated monoclonal antibodies, optimized antibody pairs, bulk recombinant proteins, and rare-target sourcing capabilities. Our team of specialists is ready to assist with customized solutions to accelerate your diagnostic projects.