Anti-GSDME (Full length+N terminal) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60443

Core Product Specifications and Parameters

Biological Background: GSDME Function and Localization

• GSDME (DFNA5/ICERE-1), encoded by the GSDME gene, is a gasdermin family pore-forming precursor protein that converts non-inflammatory apoptosis to pyroptosis.
  Related references: PMID:27281216, PMID:28459430, PMID:33852854
• Upon cleavage by caspase-3 (CASP3) or granzyme B (GZMB), the N-terminal fragment translocates to the plasma membrane, oligomerizes, and forms pores of 10–15 nm inner diameter, facilitating the release of IL-1β and IL-18 and triggering pyroptotic cell death.
  Related references: PMID:27281216, PMID:28459430, PMID:32188940, PMID:33852854
• GSDME serves as a critical switch that converts TNF- or chemotherapy-induced apoptosis to pyroptosis, and it is involved in secondary necrosis during viral infections.
  Related references: PMID:27281216, PMID:28459430, PMID:32188940, PMID:28045099
• The protein exhibits bactericidal activity and contributes to secondary necrosis downstream of the mitochondrial apoptotic pathway.
  Related references: PMID:27281216, PMID:28045099
• As a tumor suppressor, GSDME facilitates granzyme B-mediated pyroptosis in tumor cells, thereby promoting robust anti-tumor immunity and tumor regression by natural killer cells.
  Related references: PMID:21522185, PMID:32188940, PMID:31953257
• GSDME is expressed in the cochlea and many other tissues, with highest expression in the placenta; mutations cause autosomal dominant non-syndromic hearing loss (DFNA5).
  Related references: PMID:9771715
• Alternative splicing produces multiple isoforms with molecular weights of approximately 11, 35, and 55 kDa.
• Full-length GSDME localizes predominantly to the cytoplasm and cytosol; upon cleavage, the N-terminal fragment associates with the cell membrane to execute its pore-forming function.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, anticipate bands corresponding to GSDME isoforms at approximately 11, 35, and 55 kDa. Use positive controls such as cell lines treated with pyroptosis inducers (e.g., chemotherapy agents) to confirm specific detection.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimization of antigen retrieval and permeabilization conditions may be required. Validate antibody specificity using blocking peptide or tissue from knockout models where possible.
• For ELISA and other quantitative assays, consider pairing this polyclonal antibody with a matched monoclonal detection antibody for a robust sandwich assay design.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be verified for critical experiments. Running appropriate negative controls (e.g., secondary antibody alone) is recommended to assess background staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for sourcing high-quality IVD raw materials, including validated antibodies and ancillary reagents for pyroptosis and cell death research. Whether you need optimized polyclonal antibodies like this anti-GSDME pAb, complementary monoclonal antibodies, or bulk reagents for immunoassay development, we offer seamless procurement with technical guidance from concept to clinic. Count on CamelBio to bridge the gap between innovative targets and reliable diagnostic products.