Anti-GSDMD Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P57764

Core Product Specifications and Parameters

Biological Background: GSDMD Function and Localization

• GSDMD is a precursor pore‑forming protein and a key executioner of pyroptosis downstream of inflammasome activation.
  Related references: PMID:26375003 PMID:26375259 PMID:27281216
• Proteolytic cleavage by caspase‑1, ‑4, or ‑5 releases the N‑terminal domain, which oligomerizes into 10–15 nm pores at the plasma membrane.
  Related references: PMID:26375003 PMID:26375259 PMID:27281216 PMID:27418190
• These pores mediate the secretion of mature IL‑1β and IL‑18, triggering inflammatory cell death (pyroptosis).
  Related references: PMID:27281216 PMID:27418190 PMID:29898893
• The N‑terminal fragment specifically binds to acidic phospholipids such as cardiolipin, phosphatidylinositol phosphates, phosphatidic acid, and phosphatidylserine, but not neutral lipids.
  Related references: PMID:27281216 PMID:29898893 PMID:36227980
• GSDMD exerts direct bactericidal activity by forming pores on both Gram‑positive and Gram‑negative bacteria.
  Related references: PMID:27281216
• Cleavage by the allergen papain generates a fragment that selectively releases IL‑33, driving type 2 immunity.
  Related references: PMID:35794369
• A caspase‑3/7‑dependent cleavage variant in the small intestine acts as a transcription coactivator, promoting immune tolerance via CIITA and MHC‑II induction.
• GSDMD is abundantly expressed in differentiating epithelial cells of the esophagus and stomach, and localizes to cytosol, inflammasome, plasma membrane, mitochondria, and nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 346–484 of human GSDMD targeting the C‑terminal portion; therefore the antibody recognizes full‑length (~53 kDa) and the caspase‑cleaved C‑terminal fragment (~30 kDa) but not the N‑terminal pore‑forming domain.
• In Western blot, a dominant band at approximately 53 kDa is expected; upon inflammasome activation, a smaller band (~30 kDa) representing the C‑terminal cleavage product may also be visible.
• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization to detect GSDMD in its multiple subcellular locations (cytosol, membrane, mitochondria, nucleus).
• This polyclonal antibody is well suited for immunoprecipitation of full‑length GSDMD and its binding partners.
• In ELISA systems, it can serve as either a capture or detection antibody; pairing with an N‑terminal‑specific GSDMD antibody allows discrimination between intact and processed forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one‑stop partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents to support pyroptosis and inflammasome research. With this anti‑GSDMD polyclonal antibody and a broad catalog of monoclonal/polyclonal antibodies, optimized antibody pairs, and custom sourcing services, we help diagnostic developers accelerate assay development from discovery to clinical application.