Anti-GSDMD (Full length+N terminal) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9D8T2

Core Product Specifications and Parameters

Biological Background: Gasdermin-D Function and Localization

• Gasdermin-D is a pore-forming protein precursor: upon cleavage by inflammatory caspases (CASP1, CASP4/CASP11), the N-terminal fragment oligomerizes to form membrane pores, triggering pyroptosis and the release of mature interleukin-1 (IL-1β and IL-18). Related references: PMID:26375003, PMID:26375259, PMID:26611636
• The N-terminal domain specifically binds to inner leaflet lipids, including monophosphorylated and bisphosphorylated phosphatidylinositols, phosphatidic acid, and cardiolipin, while it does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine, or phosphatidylcholine. Related references: PMID:27339137, PMID:27383986
• Gasdermin-D pores (10–15 nm inner diameter) also mediate the release of mitochondrial DNA and mature caspase-7 (CASP7); extracellular N-terminal fragments can directly kill both Gram-negative and Gram-positive bacteria via lipid-binding specificity. Related references: PMID:37001519, PMID:35705808, PMID:27383986
• Under certain conditions, cleaved Gasdermin-D acts as a transcription coactivator: it translocates to the nucleus and cooperates with STAT1 to induce CIITA and MHC class II molecules, maintaining food tolerance in the small intestine. Related references: PMID:37327784
• Cleavage by allergens such as papain also activates Gasdermin-D, leading to the specific release of interleukin-33 (IL-33) and promoting a type 2 inflammatory immune response. Related references: PMID:35749514, PMID:35794369
• Subcellular localization includes cytosol, inflammasome, cell membrane, mitochondrion membrane, and nucleus, depending on the activation state.
• Tissue specificity: Highly expressed in brain endothelial cells.
• Key post-translational modifications include palmitoylation, phosphorylation, and isopeptide bond formation; classified under immunity, inflammasome, and necrosis keywords.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 80–163 of mouse cleaved GSDMD, which corresponds to part of the N-terminal pore-forming domain; this region is conserved across human, mouse, and rat, supporting broad reactivity.
• For western blot (WB), expect to detect full-length GSDMD at ~53 kDa and the cleaved N-terminal fragment at approximately 30–35 kDa upon inflammasome activation. Validate band patterns in your sample system with appropriate positive and negative controls.
• For immunofluorescence (IF/ICC), consider using inflammasome activators (e.g., LPS plus nigericin) to induce GSDMD cleavage and observe translocation from the cytosol to the plasma membrane. Co-staining with membrane or pyroptosis markers may help confirm pore formation.
• The antibody is suitable for ELISA, but optimal coating conditions and detection ranges should be empirically determined for each assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibodies, bulk reagents, and rare target procurement from concept to clinic. This anti-GSDMD polyclonal antibody is part of a broader portfolio for pyroptosis and innate immunity research, and we can also source optimized monoclonal antibody pairs, ancillary reagents, and custom immunogen design to accelerate your assay development. Whether you need small-scale pilot batches or production-scale supply, CamelBio bridges your research needs with reliable raw material sourcing.