Anti-GSDMD (Full Length+C terminal) Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9D8T2

Core Product Specifications and Parameters

Biological Background: Gasdermin-D Function and Localization

• Gasdermin-D (GSDMD) is a precursor of a pore-forming protein crucial for pyroptosis and innate immunity. It is cleaved by inflammatory caspases (CASP1, CASP4/CASP11) upon inflammasome activation, releasing the active N‑terminal fragment. Related references: PMID:26375003 PMID:26375259 PMID:26611636
• The cleaved GSDMD N‑terminal fragment translocates to the plasma membrane, binds phosphoinositides and cardiolipin, and oligomerizes into 10–15 nm pores. This permits the release of mature IL‑1β, IL‑18, and caspase‑7, thereby executing pyroptosis. Related references: PMID:27339137 PMID:27383986 PMID:29195811 PMID:29274245 PMID:35705808
• GSDMD also forms pores in mitochondrial membranes, triggering release of mtDNA, and can directly kill extracellular bacteria via its pore-forming activity. Related references: PMID:37001519 PMID:27383986
• In the upper small intestine, caspase‑3/7 cleavage produces a GSDMD fragment that acts as a transcription coactivator for STAT1, upregulating CIITA and MHC‑II to maintain food tolerance. Related references: PMID:37327784
• Allergen‑induced cleavage of GSDMD by papain drives IL‑33 release, promoting type 2 inflammatory responses. Related references: PMID:35794369 PMID:35749514
• GSDMD belongs to the gasdermin family (also known as Gsdmdc1); in mouse, it is highly expressed in brain endothelial cells and has a predicted molecular weight of ~53 kDa.
• Subcellular localization: inactive full-length GSDMD is predominantly cytosolic; upon cleavage, the N‑terminal fragment localizes to the plasma membrane, mitochondria, and is also secreted.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein of full-length mouse Gasdermin D (NP_081236.1); the antibody is expected to detect both full-length GSDMD and the C‑terminal fragment.
• For WB, full-length GSDMD migrates at ~53 kDa, the N‑terminal fragment at ~31 kDa, and the C‑terminal fragment at ~22 kDa. Validate these patterns in relevant mouse cell lines or tissues.
• In IF/ICC and IHC-P, confirm antibody specificity using appropriate controls (e.g., GSDMD‑knockout or knockdown samples).
• For ELISA, perform a standard curve with recombinant GSDMD protein to optimize detection sensitivity and range.
• Note that cross‑reactivity is reported for mouse; testing against other species may require independent validation.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting. Our anti‑GSDMD monoclonal antibody is an essential tool for pyroptosis, inflammasome signaling, and innate immunity research. As your one‑stop partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare‑target raw materials—streamlining your development from concept to clinic.