Anti-GSDMD (Full length+C terminal) Rabbit Monoclonal Antibody for WB, ELISA - P57764

Core Product Specifications and Parameters

Biological Background: GSDMD Function and Localization

• Gasdermin-D (GSDMD) is a member of the gasdermin family and serves as a precursor of a pore-forming protein critical for pyroptosis and host defense. Related references: PMID:26375003, PMID:26375259, PMID:27281216
• Upon cleavage by inflammatory caspases (CASP1/4/5), the N-terminal fragment translocates to the plasma membrane, binds inner leaflet lipids such as phosphatidylinositol phosphates, and oligomerizes to form 10-15 nm pores, releasing IL-1β and IL-18 and triggering pyroptosis. Related references: PMID:27281216, PMID:27418190, PMID:29898893
• GSDMD pores also mediate mitochondrial membrane permeabilization, leading to mtDNA release, and the N-terminal fragment exhibits direct bactericidal activity against both Gram-negative and Gram-positive bacteria. Related references: PMID:27281216
• In the small intestine, a CASP3/7-cleaved fragment acts as a transcription coactivator for STAT1, promoting CIITA and MHC class II expression to maintain food tolerance.
• GSDMD is expressed in differentiating cells of the esophagus and stomach, and its activation in brain endothelial cells can cause blood-brain barrier breakdown during excessive pyroptosis.
• Post-translational modifications include palmitoylation, phosphorylation, and ubiquitination, and it belongs to the gasdermin family characterized by a conserved N-terminal pore-forming domain.
• Cleavage by papain allergen generates a pore-forming fragment that specifically releases IL-33, driving type 2 inflammation. Related references: PMID:35794369

Experimental Guidance and Technical Tips

• The immunogen (aa 346-484) corresponds to the C-terminal portion; therefore, this antibody may recognize both full-length GSDMD and the cleaved C-terminal fragment. For Western blot, consider validating detection of endogenous protein under inflammasome-activating conditions to distinguish cleaved species.
• A band at approximately 53 kDa represents full-length GSDMD; cleavage releases the N-terminal pore-forming domain (~31 kDa) and C-terminal fragment (~22 kDa). Use appropriate controls (e.g., LPS+ATP-treated macrophages) to confirm specificity.
• For ELISA, the antibody may be suitable as a capture or detection reagent. Consider pairing with an N-terminal-specific antibody to differentially quantify full-length versus cleaved GSDMD. Optimize coating and blocking conditions empirically.
• Since GSDMD forms oligomeric pores, solubility and conformation may vary across sample types; include mild detergents and sonication in lysis buffers for efficient extraction.

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CamelBio serves as a comprehensive IVD raw‑material partner, connecting diagnostic developers and researchers with high‑quality antibodies like this GSDMD monoclonal antibody, validated for pyroptosis and inflammasome applications. Our portfolio includes ready‑to‑use antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare‑target raw materials, all supported by technical and regulatory expertise to accelerate your assay development from concept to clinic.