Anti-Gsdmc3 Rabbit Polyclonal Antibody for WB, ELISA - Q8CB12

Core Product Specifications and Parameters

Biological Background: Gasdermin-C3 Function and Localization

• Gasdermin-C3 (Gsdmc3) is a member of the gasdermin family of pore-forming proteins that play critical roles in pyroptosis and innate immunity.
• The full-length protein serves as a precursor; upon proteolytic cleavage by caspase CASP8, the N-terminal fragment is released and acquires the ability to bind membrane lipids (By similarity).
• The cleaved N-terminal moiety translocates to the plasma membrane, where it oligomerizes and forms pores of 10–15 nm in diameter, leading to membrane permeabilization and pyroptotic cell death (By similarity).
• Gasdermin-C3 localizes primarily in the cytoplasm and cytosol in its inactive state, and relocalizes to the cell membrane after activation (By similarity).
• The protein is post-translationally modified by palmitoylation, which may regulate its membrane targeting; it also contains transmembrane beta strands consistent with its pore-forming function.
• Gasdermin-C3 is implicated in necrosis, highlighting its role in lytic inflammatory cell death pathways.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal pore-forming domain (residues 2-80) of mouse Gsdmc3; this region is critical for membrane binding and oligomerization, making it a strategic target for detecting both full-length and cleaved forms.
• For Western blot, the antibody may detect full-length Gsdmc3 at ~54 kDa. Under pyroptosis-inducing conditions, a cleaved N-terminal fragment may appear at a lower molecular weight; consider using appropriate cell lysis buffers and protease inhibitors during sample preparation.
• In ELISA, this polyclonal antibody may be used to capture or detect Gsdmc3; careful optimization of coating concentrations and blocking conditions is recommended.
• Cross-reactivity is reported for mouse; for other species, validate specificity in the relevant sample system before use.
• Polyclonal nature might yield multiple bands in WB depending on sample; pre-adsorption or titration can help reduce nonspecific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to premium IVD raw materials, including this anti-Gsdmc3 antibody, tailored for pyroptosis and cell death research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Whether you need rare-target antibodies or large-scale production, CamelBio is your trusted sourcing bridge from concept to clinic.