Anti-GSDMC Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BYG8

Core Product Specifications and Parameters

Biological Background: Gasdermin-C Function and Localization

• Gasdermin-C (GSDMC), also known as melanoma-derived leucine zipper-containing extranuclear factor (MLZE), is the precursor of a pore-forming protein that triggers pyroptosis upon cleavage.
  Related references: PMID:27281216, PMID:32929201, PMID:34012073
• Cleavage of gasdermin-C by caspase CASP8 in response to death signals releases the N-terminal pore-forming moiety, which translocates to the plasma membrane.
  Related references: PMID:32929201, PMID:34012073
• The N‑terminal fragment binds strongly to membrane inner leaflet lipids and homooligomerizes to form pores of 10–15 nm inner diameter, leading to membrane permeabilization and pyroptotic cell death.
  Related references: PMID:32929201, PMID:34012073
• Under basal conditions, gasdermin-C localizes predominantly to the cytoplasm, but upon activation it inserts into the cell membrane as a transmembrane β‑strand pore.
• Tissue expression is highest in trachea and spleen, with additional expression in differentiating esophageal cells and gastric epithelium.
• The protein is subject to palmitoylation and is annotated with keywords including necrosis, lipoprotein, and transmembrane beta strand, underscoring its role in regulated cell death.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–100 of human GSDMC; therefore, the antibody may recognize both full-length (∼58 kDa) and the cleaved N‑terminal fragment. When performing Western blot, consider using lysates from caspase‑activated cells to distinguish cleavage products.
• Recommended applications include WB, IF/ICC, and ELISA. For WB, validate in cell lines with confirmed GSDMC expression, such as those derived from trachea, spleen, or gastric epithelium.
• In IF/ICC, anticipate cytoplasmic staining under resting conditions and potential membrane translocation upon pyroptotic stimulation. Optimize fixation and permeabilization protocols to preserve membrane‑bound signals.
• For ELISA, use recombinant GSDMC protein or peptide standards to establish assay sensitivity and specificity. Include appropriate controls to account for matrix effects from relevant biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering this anti-GSDMC polyclonal antibody alongside a comprehensive range of validated antibodies, antigen pairs, and ancillary reagents for pyroptosis and cell death research. Leverage our capabilities in bulk monoclonal and polyclonal antibody production, rare target sourcing, and one-stop supply chain solutions to accelerate your diagnostic development from concept to clinic. Let CamelBio bridge your raw‑material needs with technical expertise, ensuring seamless project progression.