Anti-GSDMA Monoclonal Antibody for WB, IF-P, ELISA - Q96QA5

Core Product Specifications and Parameters

Biological Background: Gasdermin-A Function and Localization

• Gasdermin-A (GSDMA), also known as Gasdermin-1, is a member of the gasdermin family of pore-forming proteins.
• It is expressed predominantly in the gastrointestinal tract and at lower levels in the skin and mammary gland; it is down-regulated in gastric cancer cells.
• GSDMA acts as a sensor of S.pyogenes infection: the bacterial protease SpeB cleaves GSDMA, releasing the N-terminal fragment.
• The N-terminal fragment binds to acidic phospholipids (e.g., cardiolipin, phosphatidylserine) on the inner leaflet of the cell membrane and oligomerizes, forming pores of 10–15 nm inner diameter. Related references: PMID:27281216, PMID:35110732, PMID:35545676
• Pore formation triggers pyroptosis, an inflammatory programmed cell death that eliminates infected cells and prevents bacterial dissemination. Related references: PMID:17471240, PMID:27281216, PMID:35110732
• Subcellularly, full-length GSDMA localizes to the cytoplasm and perinuclear region, while the cleaved N-terminal fragment translocates to the cell membrane.
• The protein has a molecular weight of approximately 49 kDa and contains transmembrane beta strands; it is modified by palmitoylation.
• GSDMA-mediated pyroptosis is critical for innate immunity at epithelial barriers, and its dysfunction may contribute to gastric cancer progression.

Experimental Guidance and Technical Tips

• For Western blotting, consider that the antibody was raised against amino acids 61–181, and may detect both full-length (~49 kDa) and the cleaved N-terminal fragment in infected or stimulated samples. Validate with appropriate controls.
• For immunofluorescence (IF-P), note that GSDMA localizes to the cytoplasm and, upon cleavage, translocates to the membrane. Optimize fixation and permeabilization to preserve this localization.
• For ELISA, the antibody can be used to detect GSDMA in cell lysates or tissue homogenates; cross-reactivity with mouse allows use in mouse models.
• As the antibody cross-reacts with mouse, it is suitable for translational studies in mouse models of infection or cancer. Consider sequence alignment to confirm epitope conservation.
• As a rabbit monoclonal antibody, it offers high specificity and reproducibility; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For GSDMA research, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support pyroptosis and epithelial barrier immunity studies. With expertise in rare target raw-material sourcing, CamelBio bridges the gap between your assay development needs and high-quality, supply-chain-secured raw materials.