Anti-GSC Monoclonal Antibody for WB, ELISA - P56915

Core Product Specifications and Parameters

Biological Background: GSC Function and Localization

• GSC encodes homeobox protein goosecoid, a DNA-binding transcription factor containing a characteristic homeobox domain.
• Regulates the expression of chordin (CHRD), a secreted BMP antagonist, important for embryonic dorsal-ventral patterning.
• Functions in spatial programming within discrete embryonic fields and lineage compartments during organogenesis (By similarity).
• Collaborates with NKX3-2 to define structural components of the middle ear, and is required for development of the entire tympanic ring (By similarity).
• Likely contributes to regulatory networks that specify neural crest cell fate and determine mesoderm cell lineages in mammals.
• Predominantly localizes to the nucleus, consistent with its role as a transcriptional regulator.
• Classified under keywords including developmental protein, DNA-binding, and dwarfism association; a 3D structure is available.

Experimental Guidance and Technical Tips

• The immunogen is an N-terminal peptide (amino acids 1–100); detectability of full-length GSC in WB can be confirmed with knockout controls or siRNA knockdown.
• For WB, a band at approximately 28 kDa is expected; titrate antibody and validate in relevant human or mouse embryonic tissue or cell models.
• In ELISA, optimize coating antigen and pairing with appropriate secondary reagents; peptide competition can help confirm specificity.
• Since cross-reactivity extends to mouse, the antibody can be applied in comparative developmental studies between human and mouse systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, spanning antibodies, antigens, and ancillary reagents. For developmental biology and organogenesis research centered on GSC, we facilitate access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk raw-material sourcing. From early-stage concept to clinic, CamelBio supports your workflow with tailored technical services and reliable supply chains.