Anti-GRSF1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12849

Core Product Specifications and Parameters

Biological Background: GRSF1 Function and Localization

• GRSF1 is a mitochondrial RNA-binding protein that regulates post-transcriptional gene expression, crucial for mitochondrial ribosome assembly and the recruitment of specific mRNAs and long non-coding RNAs (lncRNAs). Related references: PMID:23473033 PMID:23473034 PMID:29967381
• It preferentially binds to RNAs containing a 14-base G-rich element, particularly ND6 mRNA and the lncRNAs for MT-CYB and MT-ND5, which each harbor multiple consensus binding sequences. Related references: PMID:23473033 PMID:23473034
• GRSF1 participates in the mitochondrial degradosome-mediated decay of non-coding transcripts (MT-ncRNAs) and tRNA-like molecules by unwinding G-quadruplex (G4) RNA structures, facilitating their degradation. Related references: PMID:29967381
• The protein localizes predominantly to the mitochondrial matrix and cytoplasm, consistent with its roles in mitochondrial RNA metabolism.
• It is a phosphoprotein and undergoes alternative splicing, suggesting dynamic regulation in mitochondrial RNA processing.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a synthetic peptide, it may recognize multiple epitopes; consider optimizing dilution and blocking conditions for each application.
• Suitable for Western blotting (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA. For IHC-P and IF/ICC, validate in relevant tissue sections or cell lines, noting mitochondrial staining patterns due to GRSF1 localization.
• Cross-reactivity with mouse and rat allows the antibody to be used in rodent model studies; verify reactivity in your specific experimental system.
• Consider mitochondrial enrichment protocols to enhance detection in subcellular fractionation experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing by offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents—including rare targets like GRSF1 for mitochondrial research. Our one-stop platform supports diagnostic manufacturers and labs with bulk supply, custom development, and technical consulting, ensuring a seamless transition from concept to clinic. For inquiries about this or other IVD-grade products, please contact our team.