Anti-GRPEL2 Polyclonal Antibody for WB, IHC-P, ELISA - Q8TAA5

Core Product Specifications and Parameters

Biological Background: GRPEL2 Function and Localization

• GRPEL2 (GrpE protein homolog 2, mitochondrial), also designated Mt-GrpE#2, is a nuclear-encoded mitochondrial matrix protein.
• It is an essential component of the PAM (presequence translocase-associated motor) complex, which drives ATP-dependent translocation of precursor proteins from the inner membrane into the mitochondrial matrix.
• GRPEL2 acts as a nucleotide exchange factor, regulating the binding of mitochondrial HSP70 (mt-HSP70) to substrate proteins and stimulating its ATPase activity.
• It modulates the oligomeric state of mt-HSP70, promoting the interconversion between inactive oligomeric and active monomeric forms.
• The protein contains a mitochondrial transit peptide and is subjected to alternative splicing, resulting in multiple isoforms.
• Post-translational modifications include acetylation, as identified by proteomic studies.
• Keywords assigned to GRPEL2 include Acetylation, Alternative splicing, Chaperone, Mitochondrion, Transit peptide, and Reference proteome.
• The canonical isoform has a calculated molecular weight of approximately 25 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 33–225 of human GRPEL2, which corresponds to the mature protein after removal of the mitochondrial targeting sequence; this design enhances the likelihood of detecting endogenous, processed GRPEL2.
• For Western blotting, a single band near 25 kDa is expected in lysates from human, mouse, or rat cells; loading mitochondrial-enriched fractions may improve signal intensity.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary due to the mitochondrial localization; consider using formalin-fixed, paraffin-embedded tissue sections and validating with known positive controls.
• As a polyclonal antibody, lot-to-lot consistency should be verified in the relevant experimental system before large-scale studies.
• No quantitative assay data are provided; optimal dilutions and incubation conditions must be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects focused on mitochondrial biology, protein import, or molecular chaperone research, we can supply this GRPEL2 antibody in flexible quantities and coordinate the sourcing of rare or custom targets. Contact us to discuss your specific procurement needs, from small-scale validation to large-scale manufacturing.