Anti-GRPEL1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HAV7

Core Product Specifications and Parameters

Biological Background: GRPEL1 Function and Localization

• GRPEL1 encodes the mitochondrial GrpE protein homolog 1, also designated HMGE or mt-GrpE#1, a conserved chaperone essential for mitochondrial protein homeostasis.
• The protein is an essential component of the presequence translocase-associated motor (PAM) complex, which drives ATP-dependent translocation of nuclear-encoded mitochondrial precursor proteins across the inner membrane into the matrix.
• GRPEL1 functions as a nucleotide exchange factor that regulates the binding of mitochondrial HSP70 (mtHsp70) to substrate proteins, controlling the chaperone cycle within the import machinery. Related references: PMID:11311562
• It is localized to the mitochondrial matrix, consistent with its role in the late stages of protein import and folding.
• Post-translational modifications include acetylation, and the protein belongs to the GrpE chaperone family, classified under the keywords: Chaperone, Mitochondrion, Acetylation, and Reference proteome.
• Structural studies (3D-structure available) have provided insights into its mechanism, although no tissue-specific expression or antimicrobial functions have been reported for this human homolog.

Experimental Guidance and Technical Tips

• The immunogen spans almost the full-length protein (amino acids 1-217), suggesting the polyclonal antibody may recognize multiple epitopes; consider epitope specificity when interpreting results from different applications.
• For Western blot (WB), the predicted molecular weight is ~24 kDa; verify band size in relevant samples, as post-translational modifications or processing of the transit peptide could alter migration.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), mitochondrial matrix localization may require optimized fixation and permeabilization protocols to ensure antigen accessibility; co-staining with mitochondrial markers is recommended.
• For ELISA applications, use appropriate recombinant protein or peptide controls to establish standard curves and confirm antibody specificity.
• Always include positive and negative controls (e.g., cells with known GRPEL1 expression, knockout lysates, or blocking peptides) to validate specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research laboratories with critical reagents like this anti-GRPEL1 antibody. We offer a broad portfolio of validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare mitochondrial and chaperone-related targets, streamlining your procurement from discovery through clinical development.