Anti-Grp94 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14625

Core Product Specifications and Parameters

Biological Background: Endoplasmin Function and Localization

• Endoplasmin (Grp94, HSP90B1) is a member of the heat shock protein 90 (Hsp90) family and functions as an ATP-dependent molecular chaperone in the endoplasmic reticulum, modulating the Wnt pathway via LRP6 folding. Related references: PMID:23572575 PMID:39509507
• It plays a critical role in protein folding and quality control within the ER, ensuring proper maturation of client proteins such as LRP6, a coreceptor of the Wnt signaling pathway. Related references: PMID:23572575 PMID:39509507
• In concert with CNPY3, endoplasmin facilitates the folding and surface expression of Toll-like receptors, including TLR4, thereby contributing to innate immune signaling. Related references: PMID:11584270
• Endoplasmin can assist in the unconventional secretion of leaderless cargo proteins like interleukin-1β (IL-1β) by promoting their translocation into the ERGIC via TMED10. Related references: PMID:32272059
• Subcellularly, it is predominantly localized in the lumen of the endoplasmic reticulum and sarcoplasmic reticulum, and is also found in melanosomes.
• Post-translational modifications include acetylation, phosphorylation, hydroxylation, disulfide bond formation, and glycosylation, which regulate its chaperone activity.
• Also known as tumor rejection antigen 1 (gp96), endoplasmin has been studied in cancer immunotherapy due to its ability to chaperone tumor antigens.
• Keywords associated with endoplasmin include chaperone, endoplasmic reticulum, nucleotide-binding, ATP-binding, and phosphoprotein.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band of approximately 92 kDa is expected; higher molecular weight species may appear due to glycosylation, and deglycosylation experiments can help confirm specificity.
• In immunohistochemistry (IHC-P), antigen retrieval is recommended, and appropriate controls should be included.
• For immunofluorescence (IF/ICC), endoplasmin is expected to show a reticular cytoplasmic staining pattern consistent with ER localization.
• The antibody may detect human, mouse, and rat endoplasmin, but species-specific validation in the relevant model system is advised.
• Because the immunogen corresponds to a conserved region, cross-reactivity is likely, but confirmation through experimental controls is necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined source for IVD raw materials, covering all stages from concept to clinic. Our portfolio includes validated antibodies, recombinant proteins, and bulk ancillary reagents for ER chaperone and cancer immunology research. For endoplasmin-targeted projects, we offer monoclonal/polyclonal antibodies, optimized antibody pairs, and custom sourcing solutions to support your assay development and production needs.