Anti-Grp94 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P14625

Core Product Specifications and Parameters

Biological Background: Grp94 Function and Localization

• Endoplasmin (Grp94, also known as gp96, TRA1) is a 92 kDa glucose-regulated protein and a member of the Hsp90 family, functioning as an ATP-dependent molecular chaperone in the endoplasmic reticulum.
• It plays a critical role in protein folding and trafficking, including the proper folding of Toll-like receptors (TLRs) such as TLR4, in cooperation with CNPY3, ensuring their cell surface expression and innate immune function. Related references: PMID:11584270
• Grp94, together with MESD, modulates the canonical Wnt signaling pathway by promoting the folding of the coreceptor LRP6, thus influencing cell differentiation and development. Related references: PMID:23572575, PMID:39509507
• It also facilitates the unconventional secretion of leaderless cargos such as interleukin-1 (IL-1) by unfolding them and enabling TMED10-mediated translocation into the ERGIC for secretion. Related references: PMID:32272059
• Subcellularly, Grp94 resides in the lumen of the endoplasmic reticulum and sarcoplasmic reticulum, and is also found in melanosomes, reflecting its role in protein processing and transport.
• The protein undergoes various post-translational modifications including acetylation, hydroxylation, phosphorylation, and disulfide bond formation, and contains an ATP-binding domain essential for its chaperone activity.
• As a high-abundance ER resident, Grp94 is implicated in cellular stress responses and has been identified as a tumor rejection antigen, highlighting its importance in cancer immunology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 40–310 of human Grp94, targeting the N-terminal region; consider this when designing experiments with truncated or mutated proteins.
• For Western blot, a band at approximately 92 kDa is expected; validate the antibody in your specific sample system to confirm reactivity.
• This antibody has been reported to work in WB, IF/ICC, IF-P, IHC-P, and ELISA; for each application, optimization of dilution and protocol is recommended.
• Cross-reactivity is documented for human, mouse, and rat; however, due to high homology among Hsp90 family members, confirm specificity for your target in relevant species.
• Store the antibody as recommended (shipped on ice) and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk reagents, and rare-target sourcing. This Grp94 polyclonal antibody supports advanced research in protein folding, ER stress pathways, and cancer biomarker development, leveraging CamelBio’s integrated supply chain and technical expertise to accelerate your assay development from concept to clinic.