Anti-GRO alpha Polyclonal Antibody for WB, IF/ICC, ELISA - P09341

Core Product Specifications and Parameters

Biological Background: GRO alpha Function and Localization

• Growth-regulated alpha protein, also known as C-X-C motif chemokine 1 (CXCL1), melanoma growth stimulatory activity (MGSA), and neutrophil-activating protein 3 (NAP-3).
• Encoded by the CXCL1 gene (GeneID: 2919) in humans.
• It is a small, secreted cytokine belonging to the CXC chemokine family, characterized by conserved cysteine residues forming disulfide bonds (UniProt keyword: Disulfide bond).
• Exhibits potent chemotactic activity for neutrophils, playing a critical role in inflammatory responses (UniProt keyword: Inflammatory response, Cytokine).
• In vitro, processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity compared to the full-length protein.
• May function in an autocrine manner on endothelial cells, suggesting involvement in angiogenesis and wound healing.
• Subcellular localization is predominantly secreted (UniProt: Secreted).
• Post-translational modifications include signal peptide cleavage and disulfide bond formation (UniProt keyword: Signal, Disulfide bond).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1-107, which includes the signal peptide; this may allow detection of both precursor and mature forms.
• For Western blot, consider reducing conditions to resolve the 11 kDa band effectively, given the presence of disulfide bonds.
• In immunofluorescence/ICC, note that GRO alpha is secreted; optimize fixation and permeabilization to retain the target.
• ELISA application may require pairing with a suitable detection antibody; validate coating conditions and standard curves in your assay.
• Cross-reactivity is reported for human and rat; reactivity with other species should be verified individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-GRO alpha antibody supports inflammation and neutrophil biology research. Beyond this product, CamelBio assists with rare target reagent sourcing and technical consulting, helping you move from concept to clinic efficiently.