Anti-GRM8 Polyclonal Antibody for WB and ELISA - O00222

Core Product Specifications and Parameters

Biological Background: GRM8 Function and Localization

• GRM8 encodes metabotropic glutamate receptor 8, a member of group III metabotropic glutamate receptors that are coupled to inhibitory G proteins.
• The receptor is a G protein-coupled receptor (GPCR) with seven transmembrane domains, located at the cell membrane.
• Upon glutamate binding, GRM8 inhibits adenylate cyclase activity, reducing intracellular cAMP levels.
• The protein undergoes post-translational modifications including glycosylation, disulfide bond formation, and ubiquitination (isopeptide bond).
• Alternative splicing produces multiple isoforms of the receptor.
• It plays a role in sensory transduction and synaptic modulation in the central nervous system.
• Keywords from UniProt: 3D-structure, Alternative splicing, Cell membrane, Disulfide bond, G protein-coupled receptor, Glycoprotein, Isopeptide bond, Membrane, Receptor, Sensory transduction, Transmembrane, Transmembrane helix, Ubl conjugation.

Experimental Guidance and Technical Tips

• In Western blotting under reducing conditions, expect a specific band near 102 kDa corresponding to the GRM8 protein. Validate using appropriate positive and negative controls.
• For ELISA applications, the immunogen peptide can serve as a positive control to optimize coating and detection conditions.
• Because this is a polyclonal antibody, lot-to-lot consistency should be verified when using in quantitative assays.
• Consider optimizing antibody dilution and incubation parameters for the specific sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-GRM8 polyclonal antibody supports neuroscience and metabotropic receptor studies, and CamelBio can assist with sourcing additional rare-target raw materials for your diagnostic development pipeline.