Anti-GRM5 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P41594

Core Product Specifications and Parameters

Biological Background: GRM5 Function and Localization

• GRM5 encodes the metabotropic glutamate receptor 5 (mGlu5), a G protein-coupled receptor that specifically binds the excitatory neurotransmitter glutamate.
• Upon ligand binding, GRM5 activates a phosphatidylinositol-calcium second messenger system, leading to calcium mobilization and activation of downstream effectors.
• The receptor plays a pivotal role in regulating synaptic plasticity and modulating neural network activity, influencing learning and memory processes.
• GRM5 is localized to the cell membrane, featuring a seven-transmembrane domain structure typical of GPCRs.
• Post-translational modifications of GRM5 include glycosylation, disulfide bond formation, and phosphorylation, which are critical for proper folding, trafficking, and signal transduction.
• Alternative splicing of the GRM5 gene generates multiple isoforms, potentially contributing to functional diversity across different tissues and developmental stages.
• As a metabotropic glutamate receptor, GRM5 is involved in slow synaptic transmission and long-term changes in neuronal excitability.
• Dysregulation of GRM5 signaling has been implicated in various neurological and psychiatric disorders, making it a key target for neuroscience research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 870-1070); for immunofluorescence (IF-P), permeabilization of cells is required to allow antibody access.
• For Western blotting (WB), the predicted molecular weight is 132 kDa, but the protein may migrate at a higher apparent molecular weight due to glycosylation; consider using optimized gel conditions.
• The antibody shows cross-reactivity with mouse and rat GRM5, making it suitable for studies across these species; validate in the relevant tissue or cell line before large-scale experiments.
• For ELISA, the polyclonal nature provides robust detection; however, titration and optimization are recommended for each assay format.
• Store the antibody under recommended conditions (e.g., -20°C) and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials, including validated antibodies like this anti-GRM5 polyclonal antibody. With expertise in neuroscience-related targets, we offer bulk sourcing of monoclonal and polyclonal antibodies, ancillary reagents, and support for rare target procurement—helping you accelerate assay development from concept to clinical application.