Anti-GRM5 Rabbit Monoclonal Antibody for WB, ELISA - P41594

Core Product Specifications and Parameters

Biological Background: GRM5 Function and Localization

• GRM5 encodes metabotropic glutamate receptor 5, a G protein-coupled receptor (GPCR) for the major excitatory neurotransmitter glutamate.
• Ligand binding triggers signaling via guanine nucleotide-binding proteins (G proteins), activating a phosphatidylinositol-calcium second messenger system and generating a calcium-activated chloride current.
• Plays a crucial role in the regulation of synaptic plasticity and the modulation of neural network activity.
• Localized at the cell membrane, functioning as a multi-pass transmembrane receptor.
• Post-translational modifications include glycosylation, phosphorylation, methylation, and disulfide bond formation, as supported by UniProt keywords.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1100–1200 at the C-terminus of human GRM5, so the antibody likely recognizes the C-terminal region.
• For Western blotting, consider using protein lysates from brain tissue or neuronal cell lines; a band near 132 kDa is expected.
• Cross-reactivity with mouse and rat is indicated; however, validation in your specific sample system is recommended.
• In ELISA applications, the immunizing peptide or recombinant GRM5 protein may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents for diagnostic development and research. This anti-GRM5 antibody supports neuroscience and GPCR studies, and we provide bulk ordering, customized sourcing, and technical consultation to streamline your workflow from concept to clinic. Contact us for one-stop access to rare-target raw materials and assay-matched antibody pairs.