Anti-GRM4 Rabbit Polyclonal Antibody for WB and ELISA - Q14833

Core Product Specifications and Parameters

Biological Background: GRM4 Function and Localization

• GRM4 encodes metabotropic glutamate receptor 4, a G protein-coupled receptor (GPCR) that binds glutamate and primarily signals through inhibition of adenylate cyclase activity.
• It belongs to the metabotropic glutamate receptor family and is also known as mGlu4, GPRC1D, and MGLUR4.
• The receptor is localized at the cell membrane, where it functions as a transmembrane transducer involved in modulating synaptic transmission.
• GRM4 is strongly expressed in the cerebellum, with lower expression levels in the hippocampus, hypothalamus, and thalamus; no expression is detected in the liver.
• Key structural features include seven transmembrane domains, disulfide bonds, and glycosylation sites, consistent with its GPCR classification.
• Alternative splicing produces multiple isoforms, contributing to functional diversity and potential regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 33–290) of human GRM4, which is conserved across mouse and rat, supporting the reported cross-reactivity.
• For Western blot, the predicted molecular weight is 102 kDa; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In ELISA, the antibody can be used for direct or indirect detection; validate performance with relevant sample types and controls, and optimize dilution and incubation times for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

This anti-GRM4 polyclonal antibody is part of CamelBio’s comprehensive IVD raw material portfolio, supporting researchers and diagnostic developers in neuroscience and GPCR research. CamelBio provides validated antibodies, bulk reagents, and rare target sourcing, streamlining your path from concept to clinic with reliable one-stop access to critical raw materials.