Anti-GRK6 Polyclonal Antibody for WB, ELISA - P43250

Core Product Specifications and Parameters

Biological Background: GRK6 Function and Localization

• G protein-coupled receptor kinase 6 (GRK6) is a serine/threonine-protein kinase that specifically phosphorylates activated forms of G protein-coupled receptors (GPCRs), initiating receptor desensitization through β-arrestin recruitment (PubMed:19423652, PubMed:20048153). Related references: PMID:19423652, PMID:20048153

• GRK6 plays a critical role in the desensitization of D2-like dopamine receptors in the striatum and modulates chemokine receptor CXCR4 signaling, which is essential for CXCL12-induced cell chemotaxis (PubMed:20048153). Related references: PMID:20048153

• In vitro, GRK6 phosphorylates rhodopsin (RHO) and also phosphorylates the non-GPCR co-receptor LRP6 during Wnt signaling, highlighting its broader substrate specificity (PubMed:19801552). Related references: PMID:19801552

• GRK6 belongs to the GRK family of kinases and is also known as GPRK6. The gene undergoes alternative splicing, producing multiple isoforms.

• The protein is predominantly membrane-associated, consistent with its role in receptor phosphorylation at the plasma membrane.

• GRK6 is subject to post-translational modifications including palmitoylation and phosphorylation, which may regulate its activity and localization.

• Widely expressed across tissues, GRK6 contributes to ubiquitous GPCR regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 268–465 of human GRK6, covering the kinase domain; this region may ensure recognition of both full-length and potential splice variants.
• For Western blot, a band around 66 kDa is expected; however, actual migration may vary depending on sample preparation and gel conditions.
• The antibody cross-reacts with human, mouse, and rat; validate performance in your specific sample system.
• Standard blocking and antibody dilution protocols should be optimized for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated anti-GRK6 antibodies for GPCR signaling studies. Our catalog spans optimized polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents, supporting your workflow from target validation to assay development. With a focus on rare-target sourcing and bulk supply, CamelBio bridges the gap between concept and clinic.