Anti-GRK3 Rabbit Monoclonal Antibody for WB, ELISA - P35626

Core Product Specifications and Parameters

Biological Background: G Protein-Coupled Receptor Kinase 3 Function and Localization

• GRK3, also known as Beta-adrenergic receptor kinase 2 (Beta-ARK-2) or BARK2, is a serine/threonine-protein kinase belonging to the G protein-coupled receptor kinase family.
• It specifically phosphorylates activated G protein-coupled receptors (GPCRs), triggering beta-arrestin-mediated receptor internalization and desensitization. Related references: PMID:21799898
• GRK3 phosphorylates ligand-bound anaphylatoxin receptors C3AR1 and C5AR1, contributing to their desensitization, and also acts on beta-adrenergic and related receptors by similarity. Related references: PMID:21799898
• It positively regulates the ciliary smoothened (SMO) signaling pathway by phosphorylating SMO, which promotes SMO ciliary trafficking and activity (by similarity).
• Localized to both presynaptic and postsynaptic compartments, GRK3 is involved in neuronal signal modulation.
• The protein features an ATP-binding domain and is subject to ubiquitination, reflecting its regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (residues 589–688) of human GRK3, favoring specificity for the target.
• For Western blotting, the predicted molecular weight is approximately 80 kDa; consider using appropriate positive controls from human cell lines or tissues.
• In ELISA applications, validate antibody performance with the relevant antigen and experimental conditions.
• Confirm cross-reactivity in the intended human sample system, as the antibody is not tested in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies targeting key signaling molecules like GRK3. Our supply capabilities encompass monoclonal antibody development, bulk reagent provision, and rare target material sourcing—all designed to streamline your project from early research to clinical application. With expertise in GPCR signaling pathways, CamelBio is your reliable partner for accelerating assay development and biomarker discovery.