Anti-GRK2 Monoclonal Antibody [KO Validated] for WB, IF/ICC, ELISA - P25098

Core Product Specifications and Parameters

Biological Background: GRK2 Function and Localization

• GRK2 (G protein-coupled receptor kinase 2), also known as beta-adrenergic receptor kinase 1 (BARK1), is a serine/threonine kinase that specifically phosphorylates activated G protein-coupled receptors (GPCRs), initiating β-arrestin-mediated receptor internalization and desensitization (PubMed:14976224, PubMed:16179383, PubMed:19306925). Related references: PMID:14976224 PMID:16179383 PMID:19306925
• It phosphorylates the agonist-occupied form of β-adrenergic receptors (ADRB2) and related catecholamine receptors, regulating cardiomyocyte contraction rate responses and promoting receptor desensitization (PubMed:19715378). Related references: PMID:19715378
• GRK2 desensitizes anaphylatoxin receptors C3AR1 and C5AR1 upon ligand binding, modulating immune cell responses (PubMed:21799898, PubMed:23077507). Related references: PMID:21799898 PMID:23077507
• It positively regulates the ciliary smoothened (SMO) signaling pathway by phosphorylating SMO (facilitating its trafficking into the cilium) and GPR161 (promoting its ciliary export), key events in Hedgehog signaling (PubMed:39138140, PubMed:40384633). Related references: PMID:39138140 PMID:40384633
• GRK2 modulates LPAR1 signaling by competing with RALA for receptor binding, affecting signaling properties and desensitizing LPAR1/LPAR2 in a phosphorylation-independent manner (PubMed:19306925). Related references: PMID:19306925
• Subcellularly, GRK2 localizes to the cytoplasm, cell membrane, cell projections (including ciliary membrane), and synaptic compartments (postsynapse and presynapse), reflecting its diverse regulatory roles.
• Tissue expression is detected in peripheral blood leukocytes, underscoring its importance in immune and cardiovascular systems.
• Key molecular features include ATP-binding, nucleotide-binding, kinase activity, phosphorylation, and transferase activity, as annotated in UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within residues 590–689, suggesting recognition of a linear epitope; denaturing Western blotting (WB) and ELISA are expected to work well.
• KO validation indicates high specificity; consider using GRK2 knockout cell lysates as a negative control in WB to confirm band identity.
• A band at ~80 kDa is predicted; validate its migration in your sample system using appropriate molecular weight markers.
• For immunofluorescence (IF/ICC), GRK2 exhibits membrane and cytoplasmic staining; ensure adequate permeabilization and consider co-staining with organelle markers.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies in common model organisms; verify signal in relevant tissues or cell lines.
• As GRK2 is involved in GPCR desensitization, functional assays (e.g., receptor internalization or β-arrestin recruitment) may complement detection experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, providing high-quality antibodies like this anti-GRK2 monoclonal antibody for GPCR signaling research. We offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and sourcing for rare targets to support your diagnostic and research workflows. From concept to clinic, our team assists with reliable materials and technical insights to accelerate your projects.