Anti-GRK1 Polyclonal Antibody for WB, IHC-P, ELISA - Q15835

Core Product Specifications and Parameters

Biological Background: GRK1 Function and Localization

• GRK1 (G protein-coupled receptor kinase 1), also known as rhodopsin kinase (RK/RHOK), is a retina-specific serine/threonine kinase that phosphorylates light-activated rhodopsin, leading to rapid desensitization of the phototransduction cascade (PubMed:15946941). This process is essential for scotopic vision and adaptation to changing illumination. Related references: PMID:15946941
• It is a member of the GRK family of kinases and functions as a key regulator of G protein-coupled receptor signaling in visual transduction.
• GRK1 localizes to the disc membranes of photoreceptor outer segments, specifically within the cilium of rod and cone cells, where it interacts with rhodopsin.
• Post-translational modifications such as C-terminal prenylation (farnesylation) and methylation are critical for its membrane targeting and kinase activity.
• Mutations in the GRK1 gene are linked to Oguchi disease and congenital stationary night blindness, underscoring its importance in retinal health and visual function.
• Expression of GRK1 is highly restricted to retinal tissue, with strong presence in both rod and cone photoreceptors.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein corresponding to amino acids 1-160 of human GRK1, which is expected to recognize both human and rodent GRK1 based on sequence conservation.
• For Western blot analysis, a prominent band near 64 kDa is anticipated in retinal lysates; we recommend using appropriate positive controls such as human, mouse, or rat retina.
• In IHC-P, antigen retrieval conditions should be optimized; staining is likely to localize to photoreceptor outer segments, and we suggest including retina sections as positive control tissue.
• For ELISA, the antibody may be used as a capture or detection reagent; cross-reactivity with related GRK family members should be assessed empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-GRK1 polyclonal antibody supports research in vision science and retinal disorders. Contact us for customized solutions and large-scale supply to accelerate your diagnostic development.