Anti-GRK1 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q15835

Core Product Specifications and Parameters

Biological Background: GRK1 Function and Localization

• GRK1 (rhodopsin kinase) is a retina-specific serine/threonine-protein kinase belonging to the G protein-coupled receptor kinase 1 family. It is also known as G protein-coupled receptor kinase 1, RHOK, and RK.
• The primary function of GRK1 is to phosphorylate light-activated rhodopsin (RHO), initiating the rapid desensitization of the phototransduction cascade. This signal turnoff is essential for scotopic vision and allows quick adaptation to changing light conditions. Related references: PMID:15946941
• GRK1 plays a role in maintaining the structural integrity of the outer nuclear layer in the retina.
• Subcellularly, GRK1 localizes to the membrane and is concentrated in the photoreceptor outer segment, a specialized cilium, where phototransduction occurs.
• Expression of GRK1 is highly tissue-specific; it is found exclusively in the rod and cone photoreceptor cells of the retina.
• Post-translational modifications such as methylation, prenylation, and phosphorylation regulate GRK1 membrane association and activity. Mutations in the GRK1 gene are associated with congenital stationary night blindness.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-160) of human GRK1, which may allow detection of both full-length protein and potential N-terminal fragments. Validate specificity with appropriate controls (e.g., GRK1 knockout or knockdown cells/tissue).
• For western blotting, the predicted molecular weight is approximately 64 kDa. Consider using fresh retinal tissue lysates or enriched photoreceptor preparations for optimal detection.
• In immunofluorescence and immunohistochemistry, anticipate localization to photoreceptor outer segments. Paraffin-embedded retinal sections (IHC-P) should be subjected to antigen retrieval optimization.
• The antibody’s cross-reactivity with mouse and rat enables its use in common model organisms, facilitating translational vision research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for diagnostic manufacturers and research laboratories, offering a comprehensive range of IVD raw materials, technical services, and consultative support from concept to clinic. This anti-GRK1 polyclonal antibody supports investigations into retinal function, GPCR signaling, and vision disorders, aligning with CamelBio’s commitment to delivering high-quality reagents. In addition to validated polyclonal antibodies, we supply carefully matched antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets — ensuring your projects progress efficiently.