Anti-GRIN2D Rabbit Polyclonal Antibody for WB, ELISA - O15399

Core Product Specifications and Parameters

Biological Background: GRIN2D Function and Localization

• GRIN2D (Glutamate Receptor Ionotropic NMDA 2D) encodes the GluN2D subunit of NMDA receptors, also known as NMDAR2D.
• As a component of NMDA receptors, it forms heterotetrameric, ligand-gated cation channels activated by L-glutamate and co-agonists, with voltage-dependent Mg2+ block. Related references: PMID:26875626, PMID:27616483, PMID:28126851
• Each GluN2 subunit imparts distinct channel properties; GluN2D influences receptor kinetics, pH sensitivity, Ca2+ permeability, and allosteric modulation. Related references: PMID:28095420
• NMDA receptors are critical for synaptic plasticity, learning, and memory formation.
• GRIN2D is localized to the postsynaptic cell membrane.
• The protein undergoes post-translational modifications including glycosylation, phosphorylation, and methylation, and contains structural disulfide bonds.
• Mutations in GRIN2D are associated with developmental and epileptic encephalopathies (e.g., DEE46, EIEE46), highlighting its role in neurological disease.

Experimental Guidance and Technical Tips

• For Western blotting, use a reducing SDS-PAGE and appropriate blocking buffer; include a loading control and validate the antibody in your specific cell or tissue lysates.
• Given the predicted molecular weight of ~144 kDa, ensure adequate gel resolution and transfer conditions for high-molecular-weight proteins.
• For ELISA applications, consider direct or sandwich formats and perform appropriate antigen coating and detection optimization.
• As cross-reactivity is reported for human and mouse, the antibody is suitable for studies in these species; however, validate in the relevant sample system, such as brain tissue or neuronal cell cultures where GRIN2D is expressed.
• The immunogen corresponds to amino acids 330–480, a region potentially conserved across species; confirm sequence homology if using in other model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, supporting neuroscience research and immunoassay development. This GRIN2D antibody is part of our extensive catalog of validated antibodies and ancillary reagents. Contact us for bulk orders, customized antibody formats, and assistance with rare target raw-material procurement to accelerate your diagnostic and research projects.