Anti-GRIN2B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13224

Core Product Specifications and Parameters

Biological Background: GRIN2B Function and Localization

• GRIN2B encodes the glutamate receptor ionotropic NMDA 2B subunit, also known as NMDAR2B, NR2B, or hNR3. It is a key component of N-methyl-D-aspartate (NMDA) receptors, which are heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent magnesium block. Related references: PMID:24272827, PMID:24863970, PMID:26875626

• Channel activation requires simultaneous binding of L-glutamate to GluN2 subunits and glycine or D-serine to GluN1 subunits, plus membrane depolarization to relieve Mg²⁺ inhibition, opening the pore for sodium, calcium influx and potassium efflux. Related references: PMID:24272827, PMID:24863970, PMID:26875626

• GRIN2B plays essential roles in synaptic plasticity, including long-term depression (LTD) in the hippocampus, which is critical for learning and memory formation.

• Phosphorylation at Ser-1303 by DAPK1 in extrasynaptic sites enhances NMDA receptor activity and calcium influx, mediating excitotoxic neuronal death in stroke.

• GluN2B-containing NMDA receptors exhibit distinct kinetic properties, pH sensitivity, Ca²⁺ permeability, and responses to allosteric modulators compared to other GluN2 subtypes, influencing synaptic dynamics. Related references: PMID:26875626, PMID:28095420, PMID:28126851

• At the subcellular level, GRIN2B localizes to the postsynaptic cell membrane and dendrites, but is also found in late endosomes, lysosomes, and associated with the cytoskeleton.

• Tissue expression is primarily in the fronto-parieto-temporal cortex and hippocampal pyramidal cells, with lower levels in the basal ganglia, linking it to cognitive functions.

• Mutations in GRIN2B are associated with neurodevelopmental disorders, including epilepsy and intellectual disability, highlighted by its inclusion in disease variant databases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1185–1484) of human GRIN2B; the antibody may detect full-length and possibly truncated isoforms. Validate reactivity in your specific sample system.
• For Western blotting, the predicted molecular weight is approximately 166 kDa; use low-percentage polyacrylamide gels (e.g., 6–8%) and optimize transfer conditions for high molecular weight proteins.
• In IF/ICC, consider permeabilization to access the intracellular epitope. Expected staining patterns include postsynaptic membrane and dendrite labeling; confirm with subcellular markers.
• For ELISA, this polyclonal antibody can serve as a capture or detection reagent; when developing sandwich immunoassays, pair with a monoclonal antibody recognizing a distinct epitope for greater specificity.
• Cross-reactivity with mouse and rat indicates conserved epitope sequences; verify performance if using these species, as reactivity may vary by tissue context.
• This antibody has not been validated for functional studies or quantitative applications; appropriate controls and titrations are recommended for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this GRIN2B antibody. Whether you require validated antibody pairs, optimized monoclonal or polyclonal antibodies, or bulk ancillary reagents, our platform streamlines procurement from discovery to clinical application. We also specialize in rare target raw materials, such as those for neuroscience and ion channel research, ensuring you have the critical components needed for your assay development.