Anti-GRIK4 Polyclonal Antibody for WB, ELISA - Q16099

Core Product Specifications and Parameters

Biological Background: GRIK4 Function and Localization

• GRIK4 encodes the glutamate receptor ionotropic kainate 4 subunit (also known as KA1, EAA1), a member of the ionotropic glutamate receptor family.
• As a kainate-type receptor subunit, GRIK4 cannot form functional homomeric ion channels; it shows channel activity only when co-assembled with GRIK1, GRIK2, or GRIK3 subunits.
  Related references:
  PMID:8263508
• Functional kainate receptors are heterotetramers; GRIK4 requires heteromeric assembly with other kainate receptor subunits to form ligand-gated, cation-permeable ion channels.
• The receptor is localized to the cell membrane, specifically at both postsynaptic and presynaptic membranes, where it participates in excitatory synaptic transmission.
• UniProt keywords associated with GRIK4 include glycoprotein, postsynaptic cell membrane, presynaptic cell membrane, ion channel, and proteomics identification, indicating its role as a glycosylated membrane receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 21–220) of human GRIK4. Consider that this region may be subject to glycosylation or conformational epitopes; performance may vary depending on sample preparation.
• For Western blot (WB), the predicted molecular weight is approximately 107 kDa. Use appropriate gel percentage (e.g., 8% SDS-PAGE) and reducing conditions to resolve the protein.
• Since GRIK4 does not form functional homomeric channels, validation in native tissues or cells co-expressing GRIK1-3 may be necessary to confirm specificity. Endogenous expression is expected in brain regions.
• For ELISA applications, titrate the antibody and consider using peptide blocking to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your neuroscience and ion channel research by offering this anti-GRIK4 polyclonal antibody alongside a comprehensive portfolio of IVD raw materials. From validated antibody pairs to optimized monoclonal/polyclonal antibodies and bulk ancillary reagents, CamelBio streamlines your sourcing for diagnostic development and research. Whether you need rare-target raw materials or one-stop procurement solutions, CamelBio bridges the gap from concept to clinic.