Anti-GRID2 Polyclonal Antibody for WB, IF/ICC, ELISA - O43424

Core Product Specifications and Parameters

Biological Background: GRID2 Function and Localization

• GRID2 encodes glutamate receptor ionotropic delta-2 (GluD2), a member of the ionotropic glutamate receptor family structurally similar to other iGluR subunits but atypical in ligand binding.
• Although it shares the classical iGluR topology, GluD2 does not bind glutamate as its primary ligand (PubMed:34936451). Instead, it participates in non-canonical signaling complexes. Related references: PMID:34936451
• Core function: promotes synaptogenesis and mediates D-serine-dependent long-term depression (LTD) and AMPA receptor endocytosis at cerebellar parallel fiber–Purkinje cell synapses via the NRX1B–CBLN1–GRID2 triad complex (PubMed:27418511). Related references: PMID:27418511
• In the presence of neurexins and cerebellins, GluD2 forms cation-selective ion channels gated by glycine and D-serine (PubMed:34936451). Related references: PMID:34936451
• Ion channel activity can be triggered by metabotropic glutamate receptor 1 (GRM1) in Purkinje cells (PubMed:24357660, PubMed:27276689). Related references: PMID:24357660 PMID:27276689
• Subcellular localization: primarily at postsynaptic cell membranes of excitatory synapses, particularly enriched in the cerebellum.
• Features multiple post-translational modifications and structural motifs, including disulfide bonds, glycosylation, phosphorylation, and metal-binding sites, as reflected in Swiss-Prot keywords (alternative splicing, calcium, cell membrane, ion channel, ligand-gated ion channel, neurodegeneration, postsynaptic cell membrane, synapse, transmembrane).

Experimental Guidance and Technical Tips

• Immunogen design: the synthetic peptide spans amino acids 900–1007 in the intracellular C-terminal region; the antibody may recognize full-length and potential C-terminal fragments.
• Western blotting: a band near 113 kDa is expected; use cerebellar or brain lysates as positive controls. Validate with siRNA knockdown or CRISPR knockout samples if possible.
• IF/ICC: permeabilization is recommended to access the cytoplasmic epitope. Staining should localize to synaptic puncta in neuronal cultures.
• ELISA: suitable for quantitative detection; consider pre‑incubation with the immunizing peptide to verify signal specificity.
• Cross-reactivity: predicted to react with human, mouse, and rat based on sequence homology; perform your own verification in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering integrated access to validated antibodies, matched antigen pairs, and ancillary reagents – all backed by technical support from discovery to clinical application. This anti‑GRID2 polyclonal antibody is part of our neuroscience portfolio, supporting research into synaptic biology, cerebellar function, and neurological disorders. Whether you need bulk quantities, custom conjugation, or hard‑to‑find target reagents, CamelBio is your reliable bridge to accelerate assay development.