Anti-GRID1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9ULK0

Core Product Specifications and Parameters

Biological Background: GRID1 Function and Localization

• GRID1 (Glutamate receptor ionotropic, delta-1; also known as GluD1) is a member of the ionotropic glutamate receptor family, sharing structural features but not binding glutamate as a primary ligand. Related references: PMID:38060673
• It acts as a trans-synaptic adhesion molecule, forming complexes with presynaptic neurexins and cerebellins, thereby regulating NMDA and AMPA receptor activity and influencing synaptic plasticity.
• Recent studies suggest it may function as a ligand-gated cation channel in the presence of neurexins and cerebellins, gated by glycine and D-serine (by similarity). However, this channel activity is disputed. Related references: PMID:39052831
• GRID1 can be triggered by GRM1 in dopaminergic neurons to form cation-selective channels (by similarity).
• It also acts as a receptor for GABA, modulating inhibitory synaptic plasticity through non-ionotropic mechanisms. Related references: PMID:38060673
• The protein is localized to the postsynaptic cell membrane, consistent with its role in synaptic signaling.
• Key features include a molecular weight of approximately 112 kDa, presence of signal peptide, transmembrane domains, and post-translational modifications such as glycosylation and disulfide bonds.
• Alternative splicing yields multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blotting, validate the predicted band at approximately 112 kDa under reducing conditions, and consider using appropriate loading controls.
• In immunofluorescence/ICC applications, optimize fixation and permeabilization protocols, as the immunogen corresponds to an intracellular C-terminal region (aa 880-1009).
• The antibody cross-reacts with mouse and rat, making it suitable for model organism studies; confirm specificity in your experimental system.
• For ELISA, the antibody may be used with recombinant protein or peptide coatings; titration and buffer optimization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for neuroscience targets like GRID1, including validated polyclonal antibodies and bulk ancillary reagents. Our one-stop sourcing model supports diagnostic manufacturers, labs, and research institutes from concept to clinic, streamlining procurement for synaptic plasticity and receptor signaling studies.